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VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601451

VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • WB

  • mIHC

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, IHC-P, WB, mIHC

Target Molecular Weight

Predicted band size: 65 kDa

Positive Control

Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-54

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:2,000

  • WB

  • 1:5,000

  • mIHC

  • 1:2,000

Target

Function

Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.

Background References

1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan

2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.

UNIPROT

SwissProt: Q8BLE7 Mouse

SwissProt: Q9JI12 Rat

Synonyms

Differentiation associated BNPI antibody

Differentiation associated Na dependent inorganic phosphate cotransporter antibody

Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody

Differentiation-associated BNPI antibody

Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody

DNPI antibody

SLC17A6 antibody

Sodium dependent inorganic phosphate cotransporter antibody

Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody

Solute carrier family 17 member 6 antibody

Expand

Images

  • mIHC analysis of mouse cerebellum tissue (paraffin-embedded) with VGLUT2 (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>, 1/2,000) and VGLUT1 (<a href="/products/HA723217" style="font-weight: bold;text-decoration: underline;">HA723217</a>, 1/2,000). <br /><br />The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse cerebellum tissue (paraffin-embedded) with VGLUT2 (HA601451, 1/2,000) and VGLUT1 (HA723217, 1/2,000).

    The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • Application: IHC-Fr<br /><br />Species: Mouse<br />Site: hippocampus<br />Sample: Frozen section<br /><br />Antibody concentration: VGLUT2 (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>, 1/500, Mouse, green); VGLUT1 (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, 1/500, Guinea pig, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse
    Site: hippocampus
    Sample: Frozen section

    Antibody concentration: VGLUT2 (HA601451, 1/500, Mouse, green); VGLUT1 (HA601449, 1/500, Guinea pig, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br />Site: hippocampus<br />Sample: Frozen section<br /><br />Antibody concentration: VGLUT2 (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>, 1/500, Mouse, green); VGLUT1 (<a href="/products/HA601449" style="font-weight: bold;text-decoration: underline;">HA601449</a>, 1/500, Guinea pig, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat
    Site: hippocampus
    Sample: Frozen section

    Antibody concentration: VGLUT2 (HA601451, 1/500, Mouse, green); VGLUT1 (HA601449, 1/500, Guinea pig, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT2 antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT2 antibody (HA601451) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601451) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT2 antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT2 antibody (HA601451) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601451) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of VGLUT2 on different lysates with Mouse anti-VGLUT2 antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse lung tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601451" style="font-weight: bold;text-decoration: underline;">HA601451</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VGLUT2 on different lysates with Mouse anti-VGLUT2 antibody (HA601451) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse lung tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601451) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"