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HOPX / HOD Recombinant Antibody [PSH10-32] - Rat IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601484

HOPX / HOD Recombinant Antibody [PSH10-32] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

HOPX / HOD Recombinant Antibody [PSH10-32] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within human aa 1-73.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 8 kDa

Positive Control

Mouse lung tissue lysate, Mouse cerebellum tissue lysate, Rat lung tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-32

Reactivity Data

IHC-Fr WB
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • WB

  • 1:2,000

Target

Function

Homeodomain-only protein is a protein that in humans is encoded by the HOPX gene. It is an important regulator of cardiac development and a marker of hippocampal neural stem cells. The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggested that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants encoding the same protein have been observed, the full-length natures of only some have been determined.

Background References

1. Caspa Gokulan R et al. HOPX: A Unique Homeodomain Protein in Development and Tumor Suppression. Cancers (Basel). 2022 Jun

2. He S et al. HOPX is a tumor-suppressive biomarker that corresponds to T cell infiltration in skin cutaneous melanoma. Cancer Cell Int. 2023 Jun

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q8R1H0 Mouse

SwissProt: Q78ZR5 Rat

Synonyms

CAMEO antibody

heart odd homeobox 1 protein antibody

HOD antibody

Homeodomain-only protein antibody

HOP antibody

HOP homeobox antibody

HOP_HUMAN antibody

HOPX antibody

LAGY antibody

Lung cancer-associated Y protein antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (<a href="/products/HA601484" style="font-weight: bold;text-decoration: underline;">HA601484</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse cerebellum tissue lysate<br />Lane 3: Mouse liver tissue lysate (low expression)<br />Lane 4: Rat lung tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 8 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 2 minutes 41 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601484" style="font-weight: bold;text-decoration: underline;">HA601484</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of HOPX / HOD on different lysates with Rat anti-HOPX / HOD antibody (HA601484) at 1/2,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse cerebellum tissue lysate
    Lane 3: Mouse liver tissue lysate (low expression)
    Lane 4: Rat lung tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 8 kDa
    Observed band size: 14 kDa

    Exposure time: 2 minutes 41 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601484) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"