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TPH2 Recombinant Antibody [PSH04-89] - Rat IgG1 (Chimeric)

Usd: 350

Specification

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Catalog# HA601494

TPH2 Recombinant Antibody [PSH04-89] - Rat IgG1 (Chimeric)

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

TPH2 Recombinant Antibody [PSH04-89] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein with Human THP2 aa 1-490.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 56 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH04-89

Reactivity Data

WB IHC-P
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:500

Target

Function

Tryptophan hydroxylase 2 (TPH2) is an isozyme of tryptophan hydroxylase found in vertebrates. In humans, TPH2 is primarily expressed in the serotonergic neurons of the brain, with the highest expression in the raphe nucleus of the midbrain. Until the discovery of TPH2 in 2003, serotonin levels in the central nervous system were believed to be regulated by serotonin synthesis in peripheral tissues, in which tryptophan hydroxylase is the dominant form.

Background References

1. Zaniewska M et al. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. Cells. 2022 Mar

2. Liu H et al. TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. Endocrinology. 2021 Jan

Subcellular Location

Cytosol, neuron projection.

UNIPROT

SwissProt: Q8CGV2 Mouse

SwissProt: Q8CGU9 Rat

Synonyms

MGC138871 antibody

ADHD7 antibody

FLJ37295 antibody

MGC138872 antibody

Neuronal tryptophan hydroxylase antibody

NTPH antibody

TPH 2 antibody

Tph2 antibody

TPH2_HUMAN antibody

Tryptophan 5-hydroxylase 2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TPH2 on different lysates with Rat anti-TPH2 antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse kidney tissue lysate (negative) (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (negative) (20 µg/Lane)<br /><br />Predicted band size: 56 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 3 minutes 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TPH2 on different lysates with Rat anti-TPH2 antibody (HA601494) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse kidney tissue lysate (negative) (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)
    Lane 4: Rat kidney tissue lysate (negative) (20 µg/Lane)

    Predicted band size: 56 kDa
    Observed band size: 50 kDa

    Exposure time: 3 minutes 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601494) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-TPH2 antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-TPH2 antibody (HA601494) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601494) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-TPH2 antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-TPH2 antibody (HA601494) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601494) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-TPH2 antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601494" style="font-weight: bold;text-decoration: underline;">HA601494</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-TPH2 antibody (HA601494) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601494) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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