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Glutamine Synthetase Recombinant Antibody [PSH10-48] - Chicken IgY (Chimeric)

Usd: 350

Specification

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Catalog# HA601516

Glutamine Synthetase Recombinant Antibody [PSH10-48] - Chicken IgY (Chimeric)

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Glutamine Synthetase Recombinant Antibody [PSH10-48] - Chicken IgY (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Human Glutamine Synthetase aa 2-373.

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse brain tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-48

Reactivity Data

IHC-Fr WB
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgY

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • WB

  • 1:1,000-1:5,000

Target

Function

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.

Background References

1. Muthu M. et. al. GLUL Ablation Can Confer Drug Resistance to Cancer Cells via a Malate-Aspartate Shuttle-Mediated Mechanism. Cancers (Basel). 2019 Dec

2. Wang Y. et. al. GLUL Promotes Cell Proliferation in Breast Cancer. J Cell Biochem. 2017 Aug

Subcellular Location

Microsome, Cytosol, Mitochondrion, Cell membrane.

UNIPROT

SwissProt: P15105 Mouse

SwissProt: P09606 Rat

Synonyms

cell proliferation-inducing protein 59 antibody

Cgl2214 antibody

GLNA antibody

GLNA_HUMAN antibody

GLNS antibody

GLUL antibody

Glutamate ammonia ligase antibody

Glutamate decarboxylase antibody

Glutamate--ammonia ligase antibody

glutamine synthase antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: bran (P7)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Application: IHC-Fr

    Species: Mouse

    Site: bran (P7)

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

  • Western blot analysis of Glutamine Synthetase on different lysates with Chicken anti-Glutamine Synthetase antibody (<a href="/products/HA601516" style="font-weight: bold;text-decoration: underline;">HA601516</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (10 µg/Lane)<br />Lane 2: Mouse liver tissue lysate (10 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (10 µg/Lane)<br />Lane 4: Rat brain tissue lysate (10 µg/Lane)<br />Lane 5: Rat liver tissue lysate (10 µg/Lane)<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601516" style="font-weight: bold;text-decoration: underline;">HA601516</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (<a href="/products/HA1026" style="font-weight: bold;text-decoration: underline;">HA1026</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Glutamine Synthetase on different lysates with Chicken anti-Glutamine Synthetase antibody (HA601516) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate (10 µg/Lane)
    Lane 2: Mouse liver tissue lysate (10 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (10 µg/Lane)
    Lane 4: Rat brain tissue lysate (10 µg/Lane)
    Lane 5: Rat liver tissue lysate (10 µg/Lane)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601516) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"