AMACR Recombinant Antibody [PSH13-54] - Mouse IgG1 (Chimeric)
Catalog# HA601602
AMACR Recombinant Antibody [PSH13-54] - Mouse IgG1 (Chimeric)
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IHC-P
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Human
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unconjugated
Overview
Product Name
AMACR Recombinant Antibody [PSH13-54] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human AMACR aa 1-382.
Species Reactivity
Human
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Human Kidney.
Conjugation
unconjugated
Clone Number
PSH13-54
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:2,000
Target
Function
α-Methylacyl-CoA racemase (AMACR, EC 5.1.99.4) is an enzyme that in humans is encoded by the AMACR gene. In mammalian cells, the enzyme is responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. This transformation is required in order to degrade (2R)-methylacyl-CoA esters by β-oxidation, which process requires the (2S)-epimer. The enzyme is known to be localised in peroxisomes and mitochondria, both of which are known to β-oxidize 2-methylacyl-CoA esters.
Background References
1. Lerner G et al. AMACR Expression is a Potential Diagnostic Marker in Apocrine Lesions of Breast, and is Associated with High Histologic Grade and Lymph Node Metastases in Some Invasive Apocrine Breast Cancers. Clin Breast Cancer. 2023 Feb
2. Travaglino A et al. Diagnostic accuracy of HNF1beta, Napsin A and P504S/Alpha-Methylacyl-CoA Racemase (AMACR) as markers of endometrial clear cell carcinoma. Pathol Res Pract. 2022 Sep
Subcellular Location
Peroxisome, Mitochondrion.
UNIPROT
Synonyms
2 arylpropionyl CoA epimerase antibody
2 methylacyl CoA racemase antibody
2-methylacyl-CoA racemase antibody
Alpha methylacyl CoA racemase antibody
Alpha methylacyl Coenzyme A racemase antibody
Alpha methylacyl-CoA racemase deficiency, included antibody
Alpha-methylacyl-CoA racemase antibody
Amacr antibody
AMACR deficiency, included antibody
AMACR_HUMAN antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601602, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"