CD16 Recombinant Antibody [PD00-12] - Mouse IgG1 (Chimeric)
Catalog# HA601634
CD16 Recombinant Antibody [PD00-12] - Mouse IgG1 (Chimeric)
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD16 Recombinant Antibody [PD00-12] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human CD16 aa 17-208 (Extracellular).
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 29 kDa
Conjugation
unconjugated
Clone Number
PD00-12
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Tissue
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1:500
Target
Function
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CD16 (FcγRIII) is a low affinity Fc receptor that binds to IgG antibodies. Two forms of the receptor, CD16A (FcγRIIIa) and CD16B (FcγRIIIb) are present in natural killer cells, neutrophils, monocytes and activated macrophages. Antibody-dependent cell-mediated cytotoxicity (ADCC), cytokine release and microbe killing can be induced upon binding with CD16. Sconocchia, et al. demonstrated in a recent study that colorectal carcinoma patients with high CD16+ cell infiltration was associated with improved overall survival after adjusting for known prognostic factors and this association was independent from CD8+ lymphocyte infiltration and presence of metastases.
Background References
1. Cheng ZF. et. al. A novel endogenous CD16-Expressing Natural Killer Cell for cancer immunotherapy. Biochem Biophys Rep. 2021 Feb
2. Zhang J. et. al. The immune potential of decidua-resident CD16(+)CD56(+) NK cells in human pregnancy. Hum Immunol. 2021 May
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
CD 16 antibody
CD 16a antibody
CD16 antibody
CD16a antibody
CD16a antigen antibody
CD16B antibody
CD16b antigen antibody
Fc fragment of IgG antibody
Fc fragment of IgG low affinity IIIa receptor (CD16) antibody
Fc fragment of IgG low affinity IIIa receptor antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601634, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601634, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD16 Recombinant Rabbit Monoclonal Antibody [PD00-12]
Application: IHC-P,IF-Tissue,mIHC
Reactivity: Human
Conjugate: unconjugated
CD16 Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
