Progesterone Receptor Recombinant Antibody [JF0549] - Mouse IgG1 (Chimeric)
Catalog# HA601647
Progesterone Receptor Recombinant Antibody [JF0549] - Mouse IgG1 (Chimeric)
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
Progesterone Receptor Recombinant Antibody [JF0549] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within N-terminal human Progesterone Receptor.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 99 kDa
Positive Control
T-47D (Human breast ductal carcinoma cell, Human Breast cancer.
Conjugation
unconjugated
Clone Number
JF0549
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:500
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IHC-P
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1:1,000
Target
Function
PR, a protein with 946 amino acids, is a ligand-activated transcription factor member of the steroid receptor super family of nuclear receptors. The functional structure is similar to that of estrogen receptor (ER), with considerable sequence homology in the DNA-binding central domain. PR is predominantly expressed in tumours of female sex steroid responsive tissues such as the mammary gland, endometrium and the ovary. About half of the breast carcinomas are ER+/PR+. A small fraction (<5%) is ER-/PR+. About half of the non-mucinous ovarian carcinomas are also PR+. From other PR-expressing tumours, meningiomas, various pancreatic neoplasms such as solid-pseudopapillary tumour and endocrine tumours, and salivary gland neoplasms are worth mentioning. The ER and PR status has been used for over 20 years as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence. Up to 75% of ER+/PR+ breast carcinomas respond positively to endocrine treatment. ER+/PR- tumours are less responsive, and thus PR status adds information to ER-status. In combination the two predict benefit from endocrine therapy both in adjuvant setting and in advanced disease. In breast cancer predominance of one isoform, namely PR-B, is common. The majority of endometrial carcinomas express only one isoform. The applications of antibodies to PR are similar to those against ER, i.e. diagnosis of PR-positive tumours (often metastasis) and prediction of therapeutic response of breast carcinoma.
Background References
1. Bondi CD et al. The effect of estradiol, progesterone, and melatonin on estrous cycling and ovarian aromatase expression in intact female mice. Eur J Obstet Gynecol Reprod Biol 174:80-5 (2014).
2. Yu Y et al. Prostate stromal cells express the progesterone receptor to control cancer cell mobility. PLoS One 9:e92714 (2014).
Subcellular Location
Nucleus, Cytoplasm, Mitochondrion outer membrane.
UNIPROT
Synonyms
NR3C3 antibody
Nuclear receptor subfamily 3 group C member 3 antibody
PGR antibody
PR antibody
PRA antibody
PRB antibody
PRGR_HUMAN antibody
Progesterone receptor antibody
Progestin receptor form A antibody
Progestin receptor form B antibody
Images
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Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: T-47D (Human breast ductal carcinoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601647, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Breast cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601647, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Progesterone Receptor Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC
Reactivity: Human,Mouse
Conjugate: unconjugated
Progesterone receptor Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
Progesterone Receptor Rabbit Polyclonal Antibody
Application: IF-Cell,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
