Rab5 Recombinant Antibody [ST47-04] - Mouse IgG1 (Chimeric)
Catalog# HA601679
Rab5 Recombinant Antibody [ST47-04] - Mouse IgG1 (Chimeric)
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Rab5 Recombinant Antibody [ST47-04] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human Rab5 aa 166-215 / 215.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate.
Conjugation
unconjugated
Clone Number
ST47-04
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:50
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IHC-P
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1:1,000
Target
Function
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
Background References
1. Suo D et al. Coronin-1 is a neurotrophin endosomal effector that is required for developmental competition for survival. Nat Neurosci 17:36-45 (2014).
2. Simon NC & Barbieri JT Exoenzyme S ADP-ribosylates Rab5 effector sites to uncouple intracellular trafficking. Infect Immun 82:21-8 (2014).
Subcellular Location
Cell membrane, Cytoplasm, Cell projection, Membrane, Endosome.
Synonyms
RAB 5 antibody
RAB 5A antibody
RAB5A antibody
RAB5A member RAS oncogene family antibody
RAB5A_HUMAN antibody
RAS associated protein RAB5A antibody
Ras related protein Rab 5A antibody
Ras-related protein Rab-5A antibody
Images
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Western blot analysis of Rab5 on different lysates with Mouse anti-Rab5 antibody (HA601679) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane)
Lane 2: MCF7 (Human breast cancer cell) cell lysate (15 µg/Lane)
Lane 3: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate (20 µg/Lane)
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate (20 µg/Lane)
Exposure time: 4 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601679, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat Anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 24 kDa
Observed band size: 24 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: MCF7 (Human breast cancer cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601679, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon adenocarcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601679, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue:Prostate adenocarcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601679, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Rab5 Recombinant Rabbit Monoclonal Antibody [ST47-04]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Rab5 Recombinant Rabbit Monoclonal Antibody [ST47-04] - BSA and Azide free
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
