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VGluT1 Recombinant Antibody [PSH10-53] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610234

VGluT1 Recombinant Antibody [PSH10-53] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

  • WB

  • IF-Tissue

Reactivity

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

Overview

Product Name

VGluT1 Recombinant Antibody [PSH10-53] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Mouse VGLUT1 aa 1-560.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, IHC-P, WB, IF-Tissue

Target Molecular Weight

Predicted band size: 62 kDa

Positive Control

Mouse brain tissue, mouse hippocampus tissue, mouse cerebellum tissue, rat brain tissue, rat hippocampus tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH10-53

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.

Background References

1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan

2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.

UNIPROT

SwissProt: Q3TXX4 Mouse

SwissProt: Q62634 Rat

Synonyms

BNPI antibody

Brain specific Na (+) dependent inorganic phosphate cotransporter antibody

Brain specific Na dependent inorganic phosphate cotransporter antibody

Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody

Slc17a7 antibody

Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody

Solute carrier family 17 member 7 antibody

Vesicular glutamate transporter 1 antibody

VGLU1_HUMAN antibody

VGLUT 1 antibody

Expand

Images

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green); 1:500 (VGluT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red)<br /><br />Antigen retrieval: Not required

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1:500 (VGLUT1, HA601392, green); 1:500 (VGluT2, HA723218, red)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green); 1:500 (VGluT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red)<br /><br />Antigen retrieval: Not required

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500 (VGLUT1, HA601392, green); 1:500 (VGluT2, HA723218, red)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green); 1:500 (VGluT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red)<br /><br />Antigen retrieval: Not required

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1:500 (VGLUT1, HA601392, green); 1:500 (VGluT2, HA723218, red)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, green); 1:500 (VGluT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red)<br /><br />Antigen retrieval: Not required

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex

    Sample: Frozen section

    Antibody concentration: 1:500 (VGLUT1, HA601392, green); 1:500 (VGluT2, HA723218, red)

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of VGLUT1 on different lysates with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (no heat)<br />Lane 2: Mouse lung tissue lysate (no heat) (negative)<br />Lane 3: Rat brain tissue lysate (no heat)<br />Lane 4: Rat lung tissue lysate (no heat) (negative)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Western blot analysis of VGLUT1 on different lysates with Rat anti-VGLUT1 antibody (HA601392) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (no heat)
    Lane 2: Mouse lung tissue lysate (no heat) (negative)
    Lane 3: Rat brain tissue lysate (no heat)
    Lane 4: Rat lung tissue lysate (no heat) (negative)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601392) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-VGLUT1 antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-VGLUT1 antibody (HA601392) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601392) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, the same antibody clone in a different buffer formulation.<br />Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500 (VGLUT1, <a href="/products/HA601392" style="font-weight: bold;text-decoration: underline;">HA601392</a>, Rat, green); 1/500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, Rabbit, red)

    This data was developed using HA601392, the same antibody clone in a different buffer formulation.
    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500 (VGLUT1, HA601392, Rat, green); 1/500 (VGLUT2, HA723218, Rabbit, red)

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