SOX2 Recombinant Antibody [PO00-28] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610236
SOX2 Recombinant Antibody [PO00-28] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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IF-Cell
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
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unconjugated
Overview
Product Name
SOX2 Recombinant Antibody [PO00-28] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human SOX2 aa 1-317.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr, IHC-P, WB, IF-Cell
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Mouse E14.5 embryo lung tissue, mouse E14.5 embryo tissue, rat E14.5 embryo lung tissue, rat brain tissue, human trachea tissue, NCCIT cell lysate, F9 cell lysate.
Conjugation
unconjugated
Clone Number
PO00-28
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns. In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases.
Background References
1. Novak D. et. al. SOX2 in development and cancer biology. Semin Cancer Biol. 2020 Dec
2. Porter L. et. al. SOX2 and squamous cancers. Semin Cancer Biol. 2020 Dec
Subcellular Location
Nucleus.
Synonyms
ANOP3 antibody
cb236 antibody
Delta EF2a antibody
lcc antibody
MCOPS3 antibody
MGC148683 antibody
MGC2413 antibody
RGD1565646 antibody
Sex determining region Y box 2 antibody
SOX 2 antibody
ExpandImages
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This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryonic brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo lung tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo lung tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Western blot analysis of SOX2 on different lysates with Rat anti-SOX2 antibody (HA601396) at 1/2,000 dilution.
Lane 1: NCCIT cell lysate (15 µg/Lane)
Lane 2: F9 cell lysate (15 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 36 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601396) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601396, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: F9 (Mouse teratocarcinoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601396, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"