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BRN3A Recombinant Antibody [PSH11-65] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610252

BRN3A Recombinant Antibody [PSH11-65] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

BRN3A Recombinant Antibody [PSH11-65] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within Mouse BRN3A aa 1-421.

Species Reactivity

Mouse

Validated Applications

IHC-Fr, IHC-P

Molecular Weight

Predicted band size: 43 kDa

Positive Control

Mouse embryo tissue, mouse embryonic eye tissue.

Conjugation

unconjugated

Clone Number

PSH11-65

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:50

Target

Function

POU domain, class 4, transcription factor 1 (POU4F1) also known as brain-specific homeobox/POU domain protein 3A (BRN3A), homeobox/POU domain protein RDC-1 or Oct-T1 is a protein that in humans is encoded by the POU4F1 gene. BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing peripheral sensory nervous system (dorsal root ganglia, trigeminal ganglion, and hindbrain sensory ganglia), certain regions of the central nervous system, retinal neurons called ganglion cells, and in cells of the B- and T-lymphocytic lineages.

Background References

1. Nishida K et al. Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons. PLoS One. 2023 Sep

2. Heppt MV et al. HDAC2 Is Involved in the Regulation of BRN3A in Melanocytes and Melanoma. Int J Mol Sci. 2022 Jan

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: P17208 Mouse

Synonyms

Brain specific homeobox/POU domain protein 3A antibody

Brain-3A antibody

Brain-specific homeobox/POU domain protein 3A antibody

Brn 3.0 antibody

BRN 3A antibody

Brn-3A antibody

Brn3 antibody

BRN3A antibody

class 4 antibody

FLJ13449 antibody

Expand

Images

  • Immunofluorescence analysis of frozen mouse embryo tissue with Rat anti-BRN3A antibody (<a href="/products/HA610252" style="font-weight: bold;text-decoration: underline;">HA610252</a>) at 1/500 dilution.<br /><br />The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.<br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA610252" style="font-weight: bold;text-decoration: underline;">HA610252</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse embryo tissue with Rat anti-BRN3A antibody (HA610252) at 1/500 dilution.

    The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610252, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded mouse embryonic eye tissue with Rat anti-BRN3A antibody (<a href="/products/HA610252" style="font-weight: bold;text-decoration: underline;">HA610252</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610252" style="font-weight: bold;text-decoration: underline;">HA610252</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryonic eye tissue with Rat anti-BRN3A antibody (HA610252) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610252) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"