p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
Catalog# HA610274
p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
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IHC-Fr
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WB
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IF-Cell
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Mouse
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Rat
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unconjugated
Overview
Product Name
p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26] - BSA and Azide free
Antibody Type
Recombinant Rat Monoclonal Antibody
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, WB, IF-Cell
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH13-26
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-Fr | IF-Cell | |
|---|---|---|---|
| mouse |
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|
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| rat |
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|
|
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.
Background References
1. Bruno F et al. The Nerve Growth Factor Receptor (NGFR/p75(NTR)): A Major Player in Alzheimer\'s Disease. Int J Mol Sci. 2023 Feb
2. Castellini C et al. The Effect of Interaction NGF/p75(NTR) in Sperm Cells: A Rabbit Model. Cells. 2022 Mar
Subcellular Location
Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine.
Synonyms
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
ExpandImages
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This data was developed using HA601436, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: brain (medial septum)
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
This data was developed using HA601436, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: colon
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
This data was developed using HA601436, the same antibody clone in a different buffer formulation.
Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 70 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601436) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"