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p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26]

Usd: 350

Specification

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Catalog# HA601436

p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26]

Application

  • IHC-Fr

  • WB

  • IF-Cell

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26]

Antibody Type

Recombinant Rat Monoclonal Antibody

Species Reactivity

Mouse, Rat

Validated Applications

IHC-Fr, WB, IF-Cell

Molecular Weight

Predicted band size: 46 kDa

Positive Control

Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH13-26

Reactivity Data

IHC-Fr WB IF-Cell
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:200

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.

Background References

1. Bruno F et al. The Nerve Growth Factor Receptor (NGFR/p75(NTR)): A Major Player in Alzheimer\'s Disease. Int J Mol Sci. 2023 Feb

2. Castellini C et al. The Effect of Interaction NGF/p75(NTR) in Sperm Cells: A Rabbit Model. Cells. 2022 Mar

Subcellular Location

Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine.

UNIPROT

SwissProt: Q9Z0W1 Mouse

SwissProt: P07174 Rat

Synonyms

CD271 antibody

CD271 antigen antibody

Gp80 LNGFR antibody

Gp80-LNGFR antibody

Low affinity nerve growth factor receptor antibody

Low affinity neurotrophin receptor p75NTR antibody

Low-affinity nerve growth factor receptor antibody

Nerve growth factor receptor antibody

Nerve growth factor receptor TNFR superfamily member 16 antibody

NGF receptor antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: brain (medial septum)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: brain (medial septum)

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: colon<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/200<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: colon

    Sample: Frozen section

    Antibody concentration: 1/200

    Antigen retrieval: Not required

  • Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (<a href="/products/HA601436" style="font-weight: bold;text-decoration: underline;">HA601436</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 59 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601436" style="font-weight: bold;text-decoration: underline;">HA601436</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 70 kDa

    Exposure time: 59 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601436) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rat anti-p75 NGF Receptor antibody (<a href="/products/HA601436" style="font-weight: bold;text-decoration: underline;">HA601436</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-p75 NGF Receptor antibody (<a href="/products/HA601436" style="font-weight: bold;text-decoration: underline;">HA601436</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"