Cytokeratin 19 Recombinant Antibody [SA30-06] - Rat IgG1 (Chimeric) - BSA and Azide free (HA610370)
Catalog# HA610370
to Cytokeratin 19
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
FC
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cytokeratin 19 on different lysates with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: SK-Br-3 cell lysate
Lane 3: T-47D cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: Lane 1-3: 4 seconds; Lane 4: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610370) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: SK-Br-3 cell lysate
Lane 3: T-47D cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: Lane 1-3: 4 seconds; Lane 4: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610370) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.