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NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610394

NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within human NEFH aa 690-740.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 112 kDa

Positive Control

Mouse cerebellum tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

JF99-07

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500-1:2,000

  • IF-Tissue

  • 1:500

Target

Function

Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.

Background References

1. Wang J et al. Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions. Front Immunol. 2024 Dec

2. Koudonas A et al. Methylation of PCDH17 and NEFH as prognostic biomarker for nonmetastatic RCC: A cohort study. Medicine (Baltimore). 2022 Jul

Subcellular Location

Cytoplasm, cytoskeleton, Cell projection, axon.

UNIPROT

SwissProt: P12036 Human

SwissProt: P19246 Mouse

SwissProt: P16884 Rat

Synonyms

200 kDa neurofilament protein antibody

CMT2CC antibody

Nefh antibody

Neurofilament heavy polypeptide 200kDa antibody

Neurofilament heavy polypeptide antibody

Neurofilament triplet H protein antibody

NF H antibody

NF-H antibody

NFH antibody

NFH_HUMAN antibody

Images

  • Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)<br />Lane 2: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 112 kDa<br />Observed band size: 200 kDa<br /><br />Exposure time: 5 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution.

    Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)
    Lane 2: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 112 kDa
    Observed band size: 200 kDa

    Exposure time: 5 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610394) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-H antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610394) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-H antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610394" style="font-weight: bold;text-decoration: underline;">HA610394</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610394) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: brain<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: brain

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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