CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610399
CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
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IHC-Fr
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IHC-P
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IF-Cell
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Immunogen
Recombinant protein within mouse CD68 aa 1-316.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-Fr, IHC-P, IF-Cell, IF-Tissue
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH06-65
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-Fr
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1:200
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IHC-P
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1:500
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IF-Cell
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1:500
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IF-Tissue
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1:200
Target
Function
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".
Background References
1. Choudhary M et al. CD68: Potential Contributor to Inflammation and RPE Cell Dystrophy. Adv Exp Med Biol. 2023
2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
Subcellular Location
Endosome membrane, Lysosome membrane; Cell membrane.
Synonyms
CD 68 antibody
CD68 antibody
CD68 antigen antibody
CD68 molecule antibody
CD68_HUMAN antibody
DKFZp686M18236 antibody
gp11 antibody
Gp110 antibody
LAMP4 antibody
Macrophage antigen CD68 (microsialin) antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA610399) at 1/2,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane)
Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (20 µg/Lane)
Exposure time: 20 seconds; ECL: K1801
CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610399, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35 kDa
Observed band size: 60-100 kDa -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Spleen
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA610399, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Spleen
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA610399, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)/NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA610399, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: NIH/3T3 (PMID: 29631164). -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610399, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"