CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610405
CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric) - BSA and Azide free
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WB
-
IHC-P
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Human
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HA601605
含抗保成分
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unconjugated
Overview
Product Name
CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human CD21 aa 1000 to the C-terminus.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 113 kDa
Conjugation
unconjugated
Clone Number
PD00-23
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
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IHC-P
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1:4,000
Target
Function
CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CD21 plays an important role in enhancing mature B cell responses to foreign antigens.
Background References
1. Smith NA. et. al. CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. J Virol. 2020 May
2. Visentini M. et. al. CD21(low) B cells are predictive markers of new digital ulcers in systemic sclerosis. Clin Exp Immunol. 2021 Aug
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C3DR antibody
CD 21 antibody
CD21 antibody
Complement C3d receptor 2 antibody
Complement C3d receptor antibody
Complement component (3d/Epstein Barr virus) receptor 2 antibody
Complement receptor type 2 antibody
CR antibody
Cr2 antibody
CR2_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD21 on different lysates with Rabbit anti-CD21 antibody (HA610405) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cells)
Lane 2: K-562 (Human chronic myelogenous leukemia cells)
Lysates/proteins at 20 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA610405, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 113 kDa
Observed band size: 150 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lymph node
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610405, 1/4,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610405, 1/4,000, 1 hour at room temperature.
Secondary antibody: HA1023, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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