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CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)

Usd: 385

Specification

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Catalog# HA601605

CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CD21 Recombinant Antibody [PD00-23] - Rat IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human CD21 aa 1000 to the C-terminus.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 113 kDa

Conjugation

unconjugated

Clone Number

PD00-23

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:4,000

Target

Function

CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CD21 plays an important role in enhancing mature B cell responses to foreign antigens.

Background References

1. Smith NA. et. al. CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells. J Virol. 2020 May

2. Visentini M. et. al. CD21(low) B cells are predictive markers of new digital ulcers in systemic sclerosis. Clin Exp Immunol. 2021 Aug

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P20023 Human

Synonyms

C3DR antibody

CD 21 antibody

CD21 antibody

Complement C3d receptor 2 antibody

Complement C3d receptor antibody

Complement component (3d/Epstein Barr virus) receptor 2 antibody

Complement receptor type 2 antibody

CR antibody

Cr2 antibody

CR2_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD21 on different lysates with Rat anti-CD21 antibody (<a href="/products/HA601605" style="font-weight: bold;text-decoration: underline;">HA601605</a>) at 1/5,000 dilution.<br /><br />Lane 1: Raji (Human Burkitt's lymphoma cells)<br />Lane 2: K-562 (Human chronic myelogenous leukemia cells)<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601605" style="font-weight: bold;text-decoration: underline;">HA601605</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rat IgG-HRP (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 113 kDa<br />Observed band size: 150 kDa

    ☑ Relative expression (RE)

    Western blot analysis of CD21 on different lysates with Rat anti-CD21 antibody (HA601605) at 1/5,000 dilution.

    Lane 1: Raji (Human Burkitt's lymphoma cells)
    Lane 2: K-562 (Human chronic myelogenous leukemia cells)

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Negative expression of CD21 protein in K562 cell is consistent with the predicted expression pattern

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601605, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,00 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 113 kDa
    Observed band size: 150 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Lymph node <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601605" style="font-weight: bold;text-decoration: underline;">HA601605</a>, 1/4,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Lymph node
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601605, 1/4,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Spleen <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601605" style="font-weight: bold;text-decoration: underline;">HA601605</a>, 1/4,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Spleen
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601605, 1/4,000, 1 hour at room temperature.
    Secondary antibody: HA1023, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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