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PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610411

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 20 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate.

Conjugation

unconjugated

Clone Number

PSH23-76

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:5,000

  • FC

  • 1:2,000

Target

Function

The gene PARK7, also known as DJ-1, encodes a protein of the peptidase C56 family. The human gene PARK7 has 8 exons and locates at chromosome band 1p36.23. DJ-1 was shown to prevent metabolite and protein damage caused by a glycolytic metabolite. This metabolite has been suggested and confirmed to be cyclic 3-phosphoglycerate (or cyclic 3-phosphoglyceric anhydride). Catalytic efficiency of DJ-1 as a hydrolase of cyclic 3-phosphoglyceric anhydride is 10,000 times higher than other reported enzymatic activities of DJ-1. Under an oxidative condition, DJ-1 inhibits the aggregation of α-synuclein via its chaperone activity, thus functioning as a redox-sensitive chaperone and as a sensor for oxidative stress. Accordingly, DJ-1 apparently protects neurons against oxidative stress and cell death. In parallel, protein DJ-1 acts as a positive regulator of androgen receptor-dependent transcription. DJ-1 is expressed in both the neural retina and retinal pigment epithelium of mammals, where it exerts a neuroprotective role against oxidative stress under both physiological and pathological conditions.

Background References

1. Pan J et al. The Imbalance of p53-Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin-Challenged Cardiomyocytes. Adv Sci (Weinh). 2023 May

2. Qu X et al. PARK7 deficiency inhibits fatty acid beta-oxidation via PTEN to delay liver regeneration after hepatectomy. Clin Transl Med. 2022 Sep

Subcellular Location

Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum.

UNIPROT

SwissProt: Q99497 Human

Synonyms

CAP1 antibody

DJ-1 antibody

DJ1 antibody

DJ1 protein antibody

Epididymis secretory sperm binding protein Li 67p antibody

FLJ27376 antibody

FLJ34360 antibody

FLJ92274 antibody

HEL S 67p antibody

Oncogene DJ1 antibody

Expand

Images

  • Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (<a href="/products/HA610411" style="font-weight: bold;text-decoration: underline;">HA610411</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate <br />Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 2 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA610411" style="font-weight: bold;text-decoration: underline;">HA610411</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 20 kDa <br />Observed band size: 20 kDa

    Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (HA610411) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate
    Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 2 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610411, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: HeLa (Human cervix adenocarcinoma epithelial cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br />Primary antibody: <a href="/products/HA610411" style="font-weight: bold;text-decoration: underline;">HA610411</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 45 minutes at room temperature. <br /> <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA610411, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.

    Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610411" style="font-weight: bold;text-decoration: underline;">HA610411</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610411, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Flow Cytometry (Intra)<br /><br />Species: Human<br />Sample: HeLa (Human cervix adenocarcinoma epithelial cell)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.<br /><br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br />Primary antibody: <a href="/products/HA610411" style="font-weight: bold;text-decoration: underline;">HA610411</a> (1/2,000) (Red) compared with Mouse IgG1 Isotype Control (<a href="/products/HA601336" style="font-weight: bold;text-decoration: underline;">HA601336</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.
    Primary antibody: HA610411 (1/2,000) (Red) compared with Mouse IgG1 Isotype Control (HA601336, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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