iFluor™ 488 Conjugated BRG1 Recombinant Rabbit Monoclonal Antibody [SN20-03]
Catalog# HA720177F
iFluor™ 488 Conjugated BRG1 Recombinant Rabbit Monoclonal Antibody [SN20-03]
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IF-Tissue
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Human
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Mouse
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated BRG1 Recombinant Rabbit Monoclonal Antibody [SN20-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human BRG1 aa 240-280.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Tissue
Molecular Weight
Predicted band size: 185 kDa
Positive Control
Rat brain tissue.
Conjugation
iFluor™ 488
Clone Number
SN20-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:200
Target
Function
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
Background References
1. Madsen MS et al. Peroxisome proliferator-activated receptor and C/EBPa synergistically activate key metabolic adipocyte genes by assisted loading. Mol Cell Biol 34:939-54 (2014).
2. Ramos P et al. Small cell carcinoma of the ovary, hypercalcemic type, displays frequent inactivating germline and somatic mutations in SMARCA4. Nat Genet 46:427-9 (2014).
Subcellular Location
Nucleus.
Synonyms
ATP dependent helicase SMARCA4 antibody
ATP-dependent helicase SMARCA4 antibody
BAF 190 antibody
BAF190 antibody
BAF190A antibody
Brahma protein like 1 antibody
BRG1 antibody
BRG1 associated factor 190A antibody
BRG1 protein antibody
BRG1-associated factor 190A antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling BRG1 (HA720177F).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody BRG1 (HA720177F, iFluor™ 488) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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