Human IL-4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Capture) [PS00-67] (HA721230)
Catalog# HA721230
Rabbit Monoclonal to Human IL-4
Tested for
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ELISA(Cap)
Reacts with
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Human
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Cynomolgus monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of IL-4 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721230 [PS00-67] diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1%BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-4 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721231 [PS00-66] (Biotin, 1:4000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture/detect antibodies, Recombinant IL-4 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721230 [PS00-67] diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1%BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-4 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721231 [PS00-66] (Biotin, 1:4000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture/detect antibodies, Recombinant IL-4 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.