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Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]

Usd: 385

Specification

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Catalog# HA721351

Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Poliovirus Receptor aa 194-343 / 417.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HUVEC cell lysate, U87-MG cell lysate, A549 cell lysates, HUVEC.

Conjugation

unconjugated

Clone Number

JE45-53

RRID

AB_3072468

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100

Target

Function

CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that in humans is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication.

Background References

1. Wu B et al. Poliovirus receptor (PVR)-like protein cosignaling network: new opportunities for cancer immunotherapy. J Exp Clin Cancer Res. 2021 Aug

2. Li Y et al. Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury. Liver Int. 2022 Dec

Subcellular Location

Cell membrane, Secreted.

UNIPROT

SwissProt: P15151 Human

Synonyms

CD155 antibody

CD155 antigen antibody

FLJ25946 antibody

HVED antibody

mE4 antibody

NECL 5 antibody

Necl-5 antibody

Necl5 antibody

Nectin like 5 antibody

Nectin like protein 5 antibody

Expand

Images

  • Western blot analysis of Poliovirus Receptor on different lysates with Rabbit anti-Poliovirus Receptor antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/1,000 dilution.<br /><br />Lane 1: HUVEC cell lysate<br />Lane 2: U87-MG cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 4 minutes 39 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Poliovirus Receptor on different lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution.

    Lane 1: HUVEC cell lysate
    Lane 2: U87-MG cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 75 kDa

    Exposure time: 4 minutes 39 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Poliovirus Receptor on A549 cell lysates with Rabbit anti-Poliovirus Receptor antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Poliovirus Receptor on A549 cell lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HUVEC cells labeling Poliovirus Receptor with Rabbit anti-Poliovirus Receptor antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Poliovirus Receptor antibody (<a href="/products/HA721351" style="font-weight: bold;text-decoration: underline;">HA721351</a>) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HUVEC cells labeling Poliovirus Receptor with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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