E2F4 Recombinant Rabbit Monoclonal Antibody [JE40-78] (HA721374)
Catalog# HA721374
Rabbit Monoclonal to E2F4
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (HA721374) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: A549 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Raji cell lysate
Lane 7: HeLa cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: A549 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Raji cell lysate
Lane 7: HeLa cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721374) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
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