Phospho-CrkL (Y207) Recombinant Rabbit Monoclonal Antibody [JE55-58]
Catalog# HA721675
Phospho-CrkL (Y207) Recombinant Rabbit Monoclonal Antibody [JE55-58]
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WB
-
Human
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Mouse
-
Rat
-
unconjugated
Overview
Product Name
Phospho-CrkL (Y207) Recombinant Rabbit Monoclonal Antibody [JE55-58]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr207 of human CrkL protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 34 kDa
Positive Control
K-562 cell lysate.
Conjugation
unconjugated
Clone Number
JE55-58
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
Crk-like protein is a protein that in humans is encoded by the CRKL gene. v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential. CrkL together with Crk participates in the Reelin signaling cascade downstream of DAB1.
Background References
1. Guo C et al. CRKL promotes hepatocarcinoma through enhancing glucose metabolism of cancer cells via activating PI3K/Akt. J Cell Mol Med. 2021 Mar
2. Kim JH et al. Bifunctional Role of CrkL during Bone Remodeling. Int J Mol Sci. 2021 Jun
Subcellular Location
cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm, protein-containing complex.
Synonyms
Crk L antibody
Crk like protein antibody
Crk-like protein antibody
Crkl antibody
CRKL_HUMAN antibody
Crkol antibody
HGNC:2363 antibody
Oncogene CrkL antibody
V crk avian sarcoma virus CT10 oncogene homolog like antibody
v crk sarcoma virus CT10 oncogene homolog (avian) like antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-CrkL (Y207) on different lysates with Rabbit anti-Phospho-CrkL (Y207) antibody (HA721675) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: K-562 treated λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 38 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721675) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CrkL Rabbit Polyclonal Antibody
Application: WB,IF-Cell,FC,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
