PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63]
Catalog# HA721706
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human PICALM aa 1-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
HepG2 cell lysate, PC-3M cell lysate, A431 cell lysate, K-562 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast tissue, human placenta tissue, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
PSH01-63
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:50
Target
Function
Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
Background References
1. Moshkanbaryans L., Xue J., Wark J.R., Robinson P.J., Graham M.E. A Novel Sequence in AP180 and CALM Promotes Efficient Clathrin Binding and Assembly. PLoS ONE 11:E0162050-E0162050 (2016)
2. Sahlender D.A., Kozik P., Miller S.E., Peden A.A., Robinson M.S. Uncoupling the functions of CALM in VAMP sorting and clathrin-coated pit formation. PLoS ONE 8:E64514-E64514 (2013)
Subcellular Location
Cell membrane, Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus
Synonyms
CALM antibody
Clathrin assembly lymphoid myeloid leukemia antibody
Clathrin assembly lymphoid myeloid leukemia protein antibody
CLTH antibody
LAP antibody
Phosphatidylinositol-binding clathrin assembly protein antibody
PICAL_HUMAN antibody
PICALM antibody
Images
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Western blot analysis of PICALM on different lysates with Rabbit anti-PICALM antibody (HA721706) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: PC-3M cell lysate
Lane 3: A431 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 65/71 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721706) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PICALM antibody (HA721706) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721706) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-64] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
PICALM Recombinant Rabbit Monoclonal Antibody [PSH01-63] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
