Human IL-33 Recombinant Rabbit Monoclonal Antibody [PSH03-05] - BSA and Azide free (Capture)
Overview
Product Name
Human IL-33 Recombinant Rabbit Monoclonal Antibody [PSH03-05] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IL-33 aa 109-270.
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Target Molecular Weight
Predicted band size: 30.8 kDa
Positive Control
Recombinant standard Human Human IL-33 (HA210539).
Conjugation
unconjugated
Clone Number
PSH03-05
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-06] to Human Human IL-33 (Detector) (HA721936) and recombinant standard Human Human IL-33 (HA210539) as the standard. The reference range value is 37-1,000 pg/mL.
Target
Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets.
Background References
1. Schmitz J., Owyang A., Oldham E., Song Y., Murphy E., McClanahan T.K., Zurawski G., Moshrefi M., Qin J., Li X., Gorman D.M., Bazan J.F., Kastelein R.A. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor- related protein ST 2 and induces T helper type 2-associated cytokines. Immunity 23:479-490 (2005).
2. Komai-Koma M., Xu D., Li Y., McKenzie A.N., McInnes I.B., Liew F.Y. IL-33 is a chemoattractant for human Th2 cells. Eur. J. Immunol. 37:2779-2786 (2007).
3. Moussion C., Ortega N., Girard J.P. The IL-1-like cytokine IL-33 is constitutively expressed in the nucleus of endothelial cells and epithelial cells in vivo: a novel \'alarmin\'? PLoS ONE 3:E3331-E3331 (2008)
Subcellular Location
Secreted.
UNIPROT
Synonyms
C9orf26 antibody
CHROMOSOME 9 OPEN READING FRAME 26 antibody
DKFZp586H0523 antibody
DVS27 antibody
DVS27 related protein antibody
IL 1F11 antibody
IL 33 antibody
IL-1F11 antibody
IL-33 antibody
IL1F11 antibody
ExpandImages
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Sandwich ELISA analysis of human IL-33 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721935) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-33 protein starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA721936)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of IL-33 were measured in duplicates, interpolated from the IL-33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-33 concentration was determined to be 379 pg/mL in cell culture media.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IL-33 Recombinant Rabbit Monoclonal Antibody [PSH03-06] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
