Claudin 1 Recombinant Rabbit Monoclonal Antibody [JE30-33] (HA721999)
Catalog# HA721999
Rabbit Monoclonal to Claudin 1
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: Caco-2 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse skin tissue lysate (40 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Rat skin tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 19 kDa
Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721999) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Claudin 1 Recombinant Rabbit Monoclonal Antibody [JE30-33] (HA721999)
Immunogen
Synthetic peptide within human claudin 1 aa 164-211 / 211.
Host
Rabbit
Positive Control
A431 cell lysate, HUVEC cell lysate, Caco-2 cell lysate, mouse skin tissue lysate, mouse liver tissue lysate, rat skin tissue lysate, rat liver tissue lysate, human esophageal cancer tissue, human skin tissue, mouse skin tissue, rat skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE30-33
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IHC-P
-
1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Claudin 1
SYNONYMS
Claudin-1 antibody;Claudin1 antibody;CLD 1 antibody;CLD1 antibody;CLD1_HUMAN antibody;CLDN 1 antibody;Cldn1 antibody;ILVASC antibody;SEMP 1 antibody;SEMP1 antibody;Senescence associated epithelial membrane protein 1 antibody;Senescence associated epithelial membrane protein antibody;Senescence-associated epithelial membrane protein antibody
SUBCELLULAR LOCATION
Cell junction, tight junction, Cell membrane, Basolateral cell membrane.
FUNCTION
Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are composed of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
CITATIONS
-
Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation
-
WB
-
Mouse
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.