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Claudin 1 Recombinant Rabbit Monoclonal Antibody [JE30-33]

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Specification

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Catalog# HA721999

Claudin 1 Recombinant Rabbit Monoclonal Antibody [JE30-33]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Claudin 1 Recombinant Rabbit Monoclonal Antibody [JE30-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Claudin 1 aa 164-211 / 211.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 23 kDa

Positive Control

A431 cell lysate, HUVEC cell lysate, Caco-2 cell lysate, mouse skin tissue lysate, mouse liver tissue lysate, rat skin tissue lysate, rat liver tissue lysate, human esophageal cancer tissue, human skin tissue, mouse skin tissue, rat skin tissue.

Conjugation

unconjugated

Clone Number

JE30-33

RRID

AB_3096766

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

Target

Function

Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are composed of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Background References

1. Jia Z et al. Claudin1 decrease induced by 1,25-dihydroxy-vitamin D3 potentiates gefitinib resistance therapy through inhibiting AKT activation-mediated cancer stem-like properties in NSCLC cells. Cell Death Discov. 2022 Mar

2. Jia Z et al. EGFR activation-induced decreases in claudin1 promote MUC5AC expression and exacerbate asthma in mice. Mucosal Immunol. 2021 Jan

Subcellular Location

Cell junction, tight junction, Cell membrane, Basolateral cell membrane.

UNIPROT

SwissProt: O95832 Human

SwissProt: O88551 Mouse

SwissProt: P56745 Rat

Synonyms

Claudin-1 antibody

Claudin1 antibody

CLD 1 antibody

CLD1 antibody

CLD1_HUMAN antibody

CLDN 1 antibody

Cldn1 antibody

ILVASC antibody

SEMP 1 antibody

SEMP1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/2,000 dilution.<br /><br />Lane 1: Wild-type FTC-133 whole cell lysate (10 µg).<br />Lane 2/3/4: Claudin 1 knockdown FTC-133 whole cell lysate (10 µg).<br /><br />Predicted band size: 23 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.<br /><br /><a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a> was shown to specifically react with Claudin 1 in wild-type FTC-133 cells. Weakened bands were observed when Claudin 1 knockdown samples were tested. Wild-type and Claudin 1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody (HA721999) at 1/2,000 dilution.

    Lane 1: Wild-type FTC-133 whole cell lysate (10 µg).
    Lane 2/3/4: Claudin 1 knockdown FTC-133 whole cell lysate (10 µg).

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

    Exposure time: 40 seconds; ECL: K1802;4-20% SDS-PAGE gel.

    HA721999 was shown to specifically react with Claudin 1 in wild-type FTC-133 cells. Weakened bands were observed when Claudin 1 knockdown samples were tested. Wild-type and Claudin 1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721999) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Claudin 1 antibody (HA721999) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721999) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Claudin 1 antibody (HA721999) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721999) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Claudin 1 antibody (HA721999) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721999) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Claudin 1 antibody (HA721999) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721999) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/2,000 dilution.<br /><br />Lane 1: A431 cell lysate (20 µg/Lane)<br />Lane 2: HUVEC cell lysate (20 µg/Lane)<br /><br />Predicted band size: 23 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 8 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721999" style="font-weight: bold;text-decoration: underline;">HA721999</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Claudin 1 on different lysates with Rabbit anti-Claudin 1 antibody (HA721999) at 1/2,000 dilution.

    Lane 1: A431 cell lysate (20 µg/Lane)
    Lane 2: HUVEC cell lysate (20 µg/Lane)

    Predicted band size: 23 kDa
    Observed band size: 19 kDa

    Exposure time: 8 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721999) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation