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LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH05-55]

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Specification

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Catalog# HA722302

LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH05-55]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IP

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LAMP1 Recombinant Rabbit Monoclonal Antibody [PSH05-55]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein.

Species Reactivity

Mouse

Validated Applications

WB, IF-Cell, IHC-P, FC, IP, IF-Tissue, IHC-Fr

Molecular Weight

Predicted band size: 44 kDa

Positive Control

C2C12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, NIH/3T3, mouse colon tissue, mouse kidney tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

PSH05-55

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

  • IP

  • 1-2μg/sample

  • IF-Tissue

  • 1:200

  • IHC-Fr

  • 1:500-1:1,000

Target

Function

Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The human LAMP1 gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34).Immunofluorescence staining of HeLa Cells with antibody to reveal lysosomal LAMP1 in red and vimentin containing intermediate filaments in green. Nuclear DNA is seen in blue. Antibodies and image courtesy EnCor Biotechnology Inc.Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins.The LAMP-1 glycoprotein is a type I transmembrane protein which is expressed at high or medium levels in at least 76 different normal tissue cell types.It resides primarily across lysosomal membranes,and functions to provide selectins with carbohydrate ligands. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. and may also play a role in tumor cell differentiation and metastasis.

Background References

1. Chaudhry N et al. Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila. Autophagy. 2022 Oct

2. Krzystek TJ et al. HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury. Autophagy. 2023 Apr

Subcellular Location

Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane.

UNIPROT

SwissProt: P11438 Mouse

Synonyms

CD107 antigen like family member A antibody

CD107 antigen-like family member A antibody

CD107a antibody

CD107a antigen antibody

LAMP 1 antibody

LAMP-1 antibody

LAMP1 antibody

LAMP1_HUMAN antibody

LAMPA antibody

LGP120 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: RAW264.7 cell lysate treated with deglycosylation<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 100-120 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722302) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: RAW264.7 cell lysate treated with deglycosylation

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 100-120 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722302) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Dorsal root ganglion<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500<br /><br />Date by conrtesy of: Mr. Rongyao Ye, School of Basic Medical Sicences, Zhejiang University

    Application: IHC-Fr

    Species: Mouse

    Site: Dorsal root ganglion

    Sample: Frozen section

    Antibody concentration: 1: 500

    Date by conrtesy of: Mr. Rongyao Ye, School of Basic Medical Sicences, Zhejiang University

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Colon<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1: 500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Colon

    Sample: Frozen section

    Antibody concentration: 1: 500

    Antigen retrieval: Not required

  • Immunocytochemistry analysis of NIH/3T3 cells labeling LAMP1 with Rabbit anti-LAMP1 antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAMP1 antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling LAMP1 with Rabbit anti-LAMP1 antibody (HA722302) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAMP1 antibody (HA722302) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody (HA722302) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722302) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LAMP1 antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LAMP1 antibody (HA722302) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722302) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Colon<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br />Primary antibody: <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>, 1/200, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA722302, 1/200, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Mouse<br />Tissue: Kidney<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br />Primary antibody: <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>, 1/200, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA722302, 1/200, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • Flow cytometric analysis of NIH/3T3 cells labeling LAMP1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling LAMP1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722302, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • LAMP1 was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: NIH/3T3 cell lysate (input)<br />Lane 2: <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a> IP in NIH/3T3 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722302" style="font-weight: bold;text-decoration: underline;">HA722302</a> in NIH/3T3 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    LAMP1 was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA722302 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722302 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: NIH/3T3 cell lysate (input)
    Lane 2: HA722302 IP in NIH/3T3 cell lysate
    Lane 3: Rabbit IgG instead of HA722302 in NIH/3T3 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 seconds; ECL: K1801

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Citation