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ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52]

Usd: 385

Specification

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Catalog# HA722011

ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ADAMTS5 aa 251-500 / 930.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 102 kDa

Positive Control

HepG2 cell lysate, LO2 cell lysate, Huh7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human liver tissue lysate, mouse placenta tissue lysate, mouse uterus tissue lysate, HepG2, NIH/3T3.

Conjugation

unconjugated

Clone Number

PSH03-52

RRID

AB_3096522

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100-1:250

  • FC

  • 1:1,000

Target

Function

A disintegrin and metalloproteinase with thrombospondin motifs 5 also known as ADAMTS5 is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. ADAMTS5 may also have a role in the pathogenesis of human osteoarthritis.

Background References

1. Zhao SS et al. ADAMTS5 as a therapeutic target for osteoarthritis: Mendelian randomisation study. Ann Rheum Dis. 2022 Jun

2. Barallobre-Barreiro J et al. Extracellular Matrix in Heart Failure: Role of ADAMTS5 in Proteoglycan Remodeling. Circulation. 2021 Dec

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: Q9UNA0 Human

SwissProt: Q9R001 Mouse

Entrez Gene: 304135 Rat

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody

A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody

A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody

A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) antibody

A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 antibody

A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody

ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody

ADAM TS 11 antibody

ADAM TS 5 antibody

ADAM TS5 antibody

Expand

Images

  • Western blot analysis of ADAMTS5 on different lysates with Rabbit anti-ADAMTS5 antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: LO2 cell lysate<br />Lane 3: Huh7 cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: PC-12 cell lysate<br />Lane 7: Human liver tissue lysate<br />Lane 8: Mouse placenta tissue lysate<br />Lane 9: Mouse uterus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 50 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ADAMTS5 on different lysates with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: LO2 cell lysate
    Lane 3: Huh7 cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: Human liver tissue lysate
    Lane 8: Mouse placenta tissue lysate
    Lane 9: Mouse uterus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 75 kDa

    Exposure time: 50 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/250 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling ADAMTS5 with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/250 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADAMTS5 antibody (HA722011) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HepG2 cells labeling ADAMTS5.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling ADAMTS5.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722011, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of NIH/3T3 cells labeling ADAMTS5.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722011" style="font-weight: bold;text-decoration: underline;">HA722011</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling ADAMTS5.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722011, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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Citation