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CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08]

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Specification

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Catalog# HA722224

CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CARM1 aa 101-500 / 608.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 66 kDa

Positive Control

MCF7 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, EA.hy926 cell lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, human testis tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

PSH05-08

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4 (protein arginine N-methyltransferase 4), is an enzyme (EC 2.1.1.125) encoded by the CARM1 gene found in human beings, as well as many other mammals. It has a polypeptide (L) chain type that is 348 residues long, and is made up of alpha helices and beta sheets. Its main function includes catalyzing the transfer of a methyl group from S-Adenosyl methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-Adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts.

Background References

1. Suresh S et al. CARM1/PRMT4: Making Its Mark beyond Its Function as a Transcriptional Coactivator. Trends Cell Biol. 2021 May

2. Kumar S et al. CARM1 Inhibition Enables Immunotherapy of Resistant Tumors by Dual Action on Tumor Cells and T Cells. Cancer Discov. 2021 Aug

Subcellular Location

Nucleus, Cytoplasm, Chromosome.

UNIPROT

SwissProt: Q86X55 Human

SwissProt: Q9WVG6 Mouse

SwissProt: Q4AE70 Rat

Synonyms

carm1 antibody

CARM1_HUMAN antibody

Coactivator associated arginine methyltransferase 1 antibody

Coactivator-associated arginine methyltransferase 1 antibody

Histone arginine methyltransferase CARM 1 antibody

Histone arginine methyltransferase CARM1 antibody

Histone-arginine methyltransferase CARM1 antibody

PRMT 4 antibody

PRMT4 antibody

Protein arginine methyltransferase antibody

Expand

Images

  • Western blot analysis of CARM1 on different lysates with Rabbit anti-CARM1 antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 3: A431 cell lysate (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br />Lane 5: HCT 116 cell lysate (20 µg/Lane)<br />Lane 6: EA.hy926 cell lysate (20 µg/Lane)<br />Lane 7: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 8: Mouse placenta tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 66 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CARM1 on different lysates with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 3: A431 cell lysate (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)
    Lane 5: HCT 116 cell lysate (20 µg/Lane)
    Lane 6: EA.hy926 cell lysate (20 µg/Lane)
    Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 8: Mouse placenta tissue lysate (40 µg/Lane)

    Predicted band size: 66 kDa
    Observed band size: 60 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722224) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CARM1 antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CARM1 antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CARM1 antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-CARM1 antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CARM1 was immunoprecipitated from 0.2 mg HCT 116 cell lysate with CARM1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HCT 116 cell lysate (input)<br />Lane 2: <a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a> IP in HCT 116 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722224" style="font-weight: bold;text-decoration: underline;">HA722224</a> in HCT 116 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 51 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    CARM1 was immunoprecipitated from 0.2 mg HCT 116 cell lysate with CARM1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722224 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HCT 116 cell lysate (input)
    Lane 2: HA722224 IP in HCT 116 cell lysate
    Lane 3: Rabbit IgG instead of HA722224 in HCT 116 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 51 seconds; ECL: K1801

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Citation