CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08] - BSA and Azide free
Catalog# HA750981
CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08] - BSA and Azide free
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CARM1 aa 101-500 / 608.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 66 kDa
Positive Control
MCF7 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, EA.hy926 cell lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, human testis tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.
Conjugation
unconjugated
Clone Number
PSH05-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4 (protein arginine N-methyltransferase 4), is an enzyme (EC 2.1.1.125) encoded by the CARM1 gene found in human beings, as well as many other mammals. It has a polypeptide (L) chain type that is 348 residues long, and is made up of alpha helices and beta sheets. Its main function includes catalyzing the transfer of a methyl group from S-Adenosyl methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-Adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts.
Background References
1. Suresh S et al. CARM1/PRMT4: Making Its Mark beyond Its Function as a Transcriptional Coactivator. Trends Cell Biol. 2021 May
2. Kumar S et al. CARM1 Inhibition Enables Immunotherapy of Resistant Tumors by Dual Action on Tumor Cells and T Cells. Cancer Discov. 2021 Aug
Subcellular Location
Nucleus, Cytoplasm, Chromosome.
Synonyms
carm1 antibody
CARM1_HUMAN antibody
Coactivator associated arginine methyltransferase 1 antibody
Coactivator-associated arginine methyltransferase 1 antibody
Histone arginine methyltransferase CARM 1 antibody
Histone arginine methyltransferase CARM1 antibody
Histone-arginine methyltransferase CARM1 antibody
PRMT 4 antibody
PRMT4 antibody
Protein arginine methyltransferase antibody
ExpandImages
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This data was developed using HA722224, the same antibody clone in a different buffer formulation.
Western blot analysis of CARM1 on different lysates with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: HCT 116 cell lysate (20 µg/Lane)
Lane 6: EA.hy926 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: Mouse placenta tissue lysate (40 µg/Lane)
Predicted band size: 66 kDa
Observed band size: 60 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722224) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA722224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722224, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA722224, the same antibody clone in a different buffer formulation.
CARM1 was immunoprecipitated from 0.2 mg HCT 116 cell lysate with CARM1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722224 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate (input)
Lane 2: HA722224 IP in HCT 116 cell lysate
Lane 3: Rabbit IgG instead of HA722224 in HCT 116 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 51 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"