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Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-28] - BSA and Azide free (Detector)

Usd: 649

Specification

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Catalog# HA722257

Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-28] - BSA and Azide free (Detector)

Application

  • ELISA(Det)

Reactivity

  • Human

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 14.4-3,500 pg/mL

PI

  • 6.81

Conjugation

  • unconjugated

Overview

Product Name

Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-28] - BSA and Azide free (Detector)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CD200R1 aa 29-243 (Q8TD46).

Species Reactivity

Human

Validated Applications

ELISA(Det)

Molecular Weight

Predicted band size: 39 kDa

Positive Control

Recombinant standard Human CD200R1 protein (HA210572).

Conjugation

unconjugated

Clone Number

PSH05-28

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Det)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-27] to Human CD200R1 (Capture) (HA722256) and recombinant standard Human CD200R1 protein (HA210572). The reference range value is 14.4-3,500 pg/mL.

Target

Function

Cell surface transmembrane glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene. CD200R1 is expressed on the surface of myeloid cells and CD4+ T cells. It interacts with CD200 transmembrane glycoprotein that can be expressed on variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells. CD200R1 activation regulates the expression of pro-inflammatory molecules such as tumor necrosis factor (TNF-alpha), interferons, and inducible nitric oxide synthase (iNOS). This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.

Background References

1. Pannunzio B et al. CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury. Cells. 2022 May

2. Linley H et al. Reduced cutaneous CD200:CD200R1 signaling in psoriasis enhances neutrophil recruitment to skin. Immun Inflamm Dis. 2022 Jul

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q8TD46 Human

Synonyms

CD 200R antibody

CD200 cell surface glycoprotein receptor antibody

CD200 receptor 1 antibody

Cd200r1 antibody

Cell surface glycoprotein CD200 receptor 1 antibody

Cell surface glycoprotein OX2 receptor 1 antibody

Cell surface glycoprotein OX2 receptor antibody

cell surface glycoprotein receptor CD200 antibody

CRTR 2 antibody

CRTR2 antibody

Expand

Images

  • Sandwich ELISA analysis of Human CD200R1 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722256" style="font-weight: bold;text-decoration: underline;">HA722256</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD200R1 protein (<a href="/products/HA210572" style="font-weight: bold;text-decoration: underline;">HA210572</a>) starting from 3,500 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722257" style="font-weight: bold;text-decoration: underline;">HA722257</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Human CD200R1 matched pair antibodies

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722256) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD200R1 protein (HA210572) starting from 3,500 pg/ml to 0 pg/ml and detect antibody (HA722257, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • The concentrations of CD200R1 were interpolated from the CD200R1 standard curves and corrected for sample dilution.Undiluted samples are mixed serum from twenty-four volunteers 25%. The mean CD200R1 concentration was determined to be 3,702 pg/mL in mixed serum from twenty-four volunteers.

    The concentrations of CD200R1 were interpolated from the CD200R1 standard curves and corrected for sample dilution.Undiluted samples are mixed serum from twenty-four volunteers 25%. The mean CD200R1 concentration was determined to be 3,702 pg/mL in mixed serum from twenty-four volunteers.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"