Skip to content

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA722283

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]

Application

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NCOA4 aa 1-400 / 614.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 70 kDa

Positive Control

Human kidney tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

PSH05-45

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:500-1:1,500

  • IF-Tissue

  • 1:200

Target

Function

Nuclear receptor coactivator 4, also known as Androgen Receptor Activator (ARA70), is a protein that in humans is encoded by the NCOA4 gene. It plays an important role in ferritinophagy, acting as a cargo receptor, binding to the ferritin heavy chain and latching on to ATG8 on the surface of the autophagosome.

Background References

1. Santana-Codina N et al. The Role of NCOA4-Mediated Ferritinophagy in Ferroptosis. Adv Exp Med Biol. 2021

2. Wu H et al. ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4. Autophagy. 2023 Jul

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q13772 Human

Entrez Gene: 27057 Mouse

Entrez Gene: 619385 Rat

Synonyms

70 kDa androgen receptor activator antibody

70 kDa androgen receptor coactivator antibody

70 kDa AR activator antibody

70 kDa AR-activator antibody

Androgen receptor coactivator 70 kD antibody

Androgen receptor coactivator 70 kDa protein antibody

Androgen receptor-associated protein of 70 kDa antibody

ARA 70 antibody

ARA70 antibody

DKFZp762E1112 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCOA4 antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-NCOA4 antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NCOA4 antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-NCOA4 antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722283" style="font-weight: bold;text-decoration: underline;">HA722283</a>) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-NCOA4 antibody (HA722283) at 1/1,500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722283) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44] - BSA and Azide free

Application: WB

Reactivity: Human

Conjugate: unconjugated

metafield image

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-45] - BSA and Azide free

Application: IHC-P,IF-Tissue

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

NCOA4 Rabbit Polyclonal Antibody

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

NCOA4 Recombinant Rabbit Monoclonal Antibody [PSH05-44]

Application: WB

Reactivity: Human

Conjugate: unconjugated