TLR9 Recombinant Rabbit Monoclonal Antibody [JE59-91]
Catalog# HA722299
TLR9 Recombinant Rabbit Monoclonal Antibody [JE59-91]
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WB
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Human
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unconjugated
Overview
Product Name
TLR9 Recombinant Rabbit Monoclonal Antibody [JE59-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TLR9 aa 361-560 / 1,032.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 116 kDa
Positive Control
Daudi cell lysate, Raji cell lysate, RPMI 8226 cell lysate.
Conjugation
unconjugated
Clone Number
JE59-91
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Toll-like receptor 9 is a protein that in humans is encoded by the TLR9 gene. TLR9 has also been designated as CD289 (cluster of differentiation 289). It is a member of the toll-like receptor (TLR) family. TLR9 is an important receptor expressed in immune system cells including dendritic cells, macrophages, natural killer cells, and other antigen presenting cells. TLR9 is expressed on endosomes internalized from the plasma membrane, binds DNA (preferentially DNA containing unmethylated CpGs of bacterial or viral origin), and triggers signaling cascades that lead to a pro-inflammatory cytokine response. Cancer, infection, and tissue damage can all modulate TLR9 expression and activation. TLR9 is also an important factor in autoimmune diseases, and there is active research into synthetic TLR9 agonists and antagonists that help regulate autoimmune inflammation.
Background References
1. Saber MM et al. TLR9: A friend or a foe. Life Sci. 2022 Oct
2. Fehri E et al. TLR9 and Glioma: Friends or Foes? Cells. 2022 Dec
Subcellular Location
Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle, phagosome.
UNIPROT
Synonyms
CD 289 antibody
CD289 antibody
TLR 9 antibody
TLR9 antibody
TLR9_HUMAN antibody
Toll like receptor 9 antibody
Toll like receptor 9 isoform A precursor antibody
Toll like receptor 9 isoform B antibody
Toll-like receptor 9 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of TLR9 on different lysates with Rabbit anti-TLR9 antibody (HA722299) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate
Lane 3: THP-1 cell lysate (negative)
Lane 4: RPMI 8226 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722299) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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