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EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE53-79]

Usd: 385

Specification

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Catalog# HA722419

EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE53-79]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

EDG3 / S1P3 Recombinant Rabbit Monoclonal Antibody [JE53-79]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide corresponding to Human EDG3/S1P3 aa 30-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HepG2 cell lysate, 293T cell lysate, PC-12 cell lysate, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JE53-79

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Sphingosine-1-phosphate receptor 3 also known as S1PR3 is a human gene which encodes a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). Hence this receptor is also known as S1P3. This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.

Background References

1. Fan X et al. Recent advances of the function of sphingosine 1-phosphate (S1P) receptor S1P3. J Cell Physiol. 2021 Mar

2. Magrassi L et al. EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas. Cancer Lett. 2010 Apr

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q99500 Human

SwissProt: Q9Z0U9 Mouse

Entrez Gene: 306792 Rat

Synonyms

EDG 3 antibody

EDG3 antibody

Endothelial differentiation G protein coupled receptor 3 antibody

Endothelial differentiation G-protein coupled receptor 3 antibody

Endothelial differentiation gene 3 antibody

Endothelial differentiation sphingolipid G protein coupled receptor 3 antibody

FLJ37523 antibody

FLJ93220 antibody

G protein coupled receptor endothelial differentiation gene 3 antibody

LPB 3 antibody

Expand

Images

  • Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate, 20 µg/Lane<br />Lane 2: 293T cell lysate, 20 µg/Lane<br />Lane 3: PC-12 cell lysate, 20 µg/Lane<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EDG3 / S1P3 on different lysates with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate, 20 µg/Lane
    Lane 2: 293T cell lysate, 20 µg/Lane
    Lane 3: PC-12 cell lysate, 20 µg/Lane

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 21 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722419) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722419" style="font-weight: bold;text-decoration: underline;">HA722419</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-EDG3 / S1P3 antibody (HA722419) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722419) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Conjugate: unconjugated