ANXA6 Recombinant Rabbit Monoclonal Antibody [PSH05-82]
Catalog# HA722481
ANXA6 Recombinant Rabbit Monoclonal Antibody [PSH05-82]
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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HA751030
不含抗保成分
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unconjugated
Overview
Product Name
ANXA6 Recombinant Rabbit Monoclonal Antibody [PSH05-82]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ANXA6 aa 1-673.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IP
Molecular Weight
Predicted band size: 76 kDa
Positive Control
Jurkat cell lysate, Raji cell lysate, THP-1 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, L6 cell lysate, mouse pancreas tissue lysate, mouse liver tissue lysate, rat heart tissue lysate, rat liver tissue lysate, human liver tissue, human lung cancer tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue.
Conjugation
unconjugated
Clone Number
PSH05-82
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:2,000-1:10,000
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IF-Tissue
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1:500-1:2,000
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IP
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1-2μg/sample
Target
Function
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.
Background References
1. Cao J et al. ANXA6: a key molecular player in cancer progression and drug resistance. Discov Oncol. 2023 May
2. Sheng Z et al. SUMOylation of AnxA6 facilitates EGFR-PKCalpha complex formation to suppress epithelial cancer growth. Cell Commun Signal. 2023 Aug
Subcellular Location
Cytoplasm.
Synonyms
67 kDa calelectrin antibody
Annexin A6 antibody
Annexin VI antibody
Annexin VI p68 antibody
Annexin-6 antibody
AnnexinA6 antibody
AnnexinVI antibody
ANX 6 antibody
ANX A6 antibody
ANX6 antibody
ExpandImages
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Western blot analysis of ANXA6 on different lysates with Rabbit anti-ANXA6 antibody (HA722481) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: PC-3M cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HeLa cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: L6 cell lysate (20 µg/Lane)
Lane 10: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 11: Mouse liver tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-13 (left): 10 seconds; Lane 1-13 (right): 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722481) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ANXA6 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722481 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722481 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA722481 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722481 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 14 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ANXA6 antibody (HA722481) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722481) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ANXA6 antibody (HA722481) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722481) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ANXA6 antibody (HA722481) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722481) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-ANXA6 antibody (HA722481) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722481) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ANXA6 antibody (HA722481) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722481) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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