ANXA6 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-82] (HA751030)
Catalog# HA751030
Rabbit Monoclonal to ANXA6
Tested for
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WB
-
IHC-P
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IF-Tissue
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ANXA6 on different lysates with Rabbit anti-ANXA6 antibody (HA751030) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: PC-3M cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HeLa cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: L6 cell lysate (20 µg/Lane)
Lane 10: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 11: Mouse liver tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-13 (left): 10 seconds; Lane 1-13 (right): 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751030) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: PC-3M cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HeLa cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: L6 cell lysate (20 µg/Lane)
Lane 10: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 11: Mouse liver tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-13 (left): 10 seconds; Lane 1-13 (right): 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751030) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.