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BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]

Usd: 385

Specification

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Catalog# HA722693

BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human BMP2 aa 51-100 / 396.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 45 kDa

Positive Control

Caco-2 cell lysate, Saos-2 cell lysate, LNCaP cell lysate, HeLa cell lysate, Human lung tissue lysate, Human liver tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

JE10-29

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. Like many other proteins from the BMP family, BMP-2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2 may be involved in white adipogenesis and may have metabolic effects.

Background References

1. Yang Z et al. Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regeneration. J Nanobiotechnology. 2023 Apr

2. Li TT et al. BMP2 as a promising anticancer approach: functions and molecular mechanisms. Invest New Drugs. 2022 Dec

Subcellular Location

Secreted.

UNIPROT

SwissProt: P12643 Human

SwissProt: P21274 Mouse

SwissProt: P49001 Rat

Synonyms

BDA2 antibody

BMP-2 antibody

BMP-2A antibody

Bmp2 antibody

BMP2_HUMAN antibody

BMP2A antibody

Bone morphogenetic protein 2 antibody

Bone morphogenetic protein 2A antibody

Images

  • Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (<a href="/products/HA722693" style="font-weight: bold;text-decoration: underline;">HA722693</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: Saos-2 cell lysate<br />Lane 3: LNCaP cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: Human lung tissue lysate<br />Lane 6: Human liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722693" style="font-weight: bold;text-decoration: underline;">HA722693</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (HA722693) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: Saos-2 cell lysate
    Lane 3: LNCaP cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: Human lung tissue lysate
    Lane 6: Human liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 38 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722693) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (<a href="/products/HA722693" style="font-weight: bold;text-decoration: underline;">HA722693</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722693" style="font-weight: bold;text-decoration: underline;">HA722693</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (HA722693) at 1/1,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 38 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722693) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

BMP2 Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated