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smooth muscle Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody [JE60-21]

Usd: 385

Specification

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Catalog# HA722863

smooth muscle Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody [JE60-21]

Application

  • WB

  • IHC-P

  • mIHC

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

smooth muscle Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody [JE60-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human smooth muscle Myosin heavy chain 11 aa 1-50 / 1,972.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, mIHC, IF-Tissue

Molecular Weight

Predicted band size: 227 kDa

Positive Control

Mouse small intestine tissue lysate, rat ovary tissue lysate, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

JE60-21

Reactivity Data

WB IHC-P mIHC IF-Tissue
Human
Not Recommended
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • mIHC

  • 1:200

  • IF-Tissue

  • 1:500

Target

Function

The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. A chromosomal rearrangement involving this gene is associated with acute myeloid leukemia of the M4Eo subtype. Mutations in this gene are associated with visceral myopathy, megacystis-microcolon-intestinal hypoperistalsis syndrome 2, and familial thoracic aortic aneurysm 4.

Background References

1. Boreham MK, Miller RT, Schaffer JI, Word RA. Smooth muscle myosin heavy chain and caldesmon expression in the anterior vaginal wall of women with and without pelvic organ prolapse. Am J Obstet Gynecol. 2001 Oct;185(4):944-52. doi: 10.1067/mob.2001.117342. PMID: 11641683.

2. Li M, Li S, Rao Y, Cui S, Gou K. Loss of smooth muscle myosin heavy chain results in the bladder and stomach developing lesion during foetal development in mice. J Genet. 2018 Jun;97(2):469-475. PMID: 29932067.

Subcellular Location

Melanosome.

UNIPROT

SwissProt: P35749 Human

SwissProt: O08638 Mouse

SwissProt: Q63862 Rat

Synonyms

AAT4 antibody

DKFZp686D10126 antibody

DKFZp686D19237 antibody

FAA4 antibody

FLJ35232 antibody

MGC126726 antibody

MGC32963 antibody

MYH 11 antibody

MYH11 antibody

MYH11_HUMAN antibody

Expand

Images

  • Western blot analysis of smooth muscle Myosin heavy chain 11 on different lysates with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1,000 dilution.<br /> <br />Lane 1: mouse small intestine tissue lysate (40 µg/Lane)<br />Lane 2: rat ovary tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 227 kDa<br />Observed band size: 227 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of smooth muscle Myosin heavy chain 11 on different lysates with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/1,000 dilution.

    Lane 1: mouse small intestine tissue lysate (40 µg/Lane)
    Lane 2: rat ovary tissue lysate (40 µg/Lane)

    Predicted band size: 227 kDa
    Observed band size: 227 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722863) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-smooth muscle Myosin heavy chain 11 antibody (HA722863) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722863) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: Immunofluorescence (IF-tissue)<br /><br />Species: Human<br />Tissue: small intestine<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× PBS<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA722863" style="font-weight: bold;text-decoration: underline;">HA722863</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: small intestine
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× PBS
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA722863, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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