HRP Conjugated Human PD-L2 Recombinant Rabbit Monoclonal Antibody [PSH09-82] - Detector
Overview
Product Name
HRP Conjugated Human PD-L2 Recombinant Rabbit Monoclonal Antibody [PSH09-82] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PD-L2 Protein aa 20-220 (HA210599)
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human PD-L2 Protein (HA210599).
Conjugation
HRP
Clone Number
PSH09-82
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH09-80] to Human PD-L2 antibody (Capture) (HA723145) or Rabbit monoclonal [PSH09-81] to Human PD-L2 antibody (Capture) (HA723146) and Recombinant Human PD-L2 protein (HA210599) as the standard.
Target
Function
Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis. Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Background References
1. Tseng S.-Y., Otsuji M., Gorski K., Huang X., Slansky J.E., Pai S.I., Shalabi A., Shin T., Pardoll D.M., Tsuchiya H. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J. Exp. Med. 193:839-846 (2001)
2. Latchman Y., Wood C.R., Chernova T., Chaudhary D., Borde M., Chernova I., Iwai Y., Long A.J., Brown J.A., Freeman G.J. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat. Immunol. 2:261-268 (2001)
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
B7 dendritic cell molecule antibody
B7-DC antibody
B7DC antibody
bA574F11.2 antibody
Btdc antibody
Butyrophilin B7 DC antibody
Butyrophilin B7-DC antibody
Butyrophilin B7DC antibody
CD 273 antibody
CD273 antibody
ExpandImages
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Sandwich ELISA analysis of Human PD-L2 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723146) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PD-L2 (HA210599) starting from 111.11 ng/ml to 0 ng/ml and detect antibody (HA723373H, HRP, 0.01 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Human PD-L2 Recombinant Rabbit Monoclonal Antibody [PSH09-80] - BSA and Azide free (Capture)
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Conjugate: unconjugated
