MEK4 / MKK4 Recombinant Rabbit Monoclonal Antibody [PSH11-99]
Catalog# HA723412
MEK4 / MKK4 Recombinant Rabbit Monoclonal Antibody [PSH11-99]
-
WB
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
Green monkey
-
HA751432
不含抗保成分
-
unconjugated
Overview
Product Name
MEK4 / MKK4 Recombinant Rabbit Monoclonal Antibody [PSH11-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human MEK4 / MKK4 aa 50-399.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 44 kDa
Positive Control
293T cell lysate, HeLa cell lysate, A431 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue, human skeletal muscle tissue, 293T.
Conjugation
unconjugated
Clone Number
PSH11-99
Reactivity Data
| WB | IHC-P | FC | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IHC-P
-
1:200
-
FC
-
1:1,000
Target
Function
Dual-specificity mitogen-activated protein kinase kinase 4 is an enzyme that in humans is encoded by the MAP2K4 gene. MAP2K4 encodes a dual-specificity kinase that belongs to the Ser/Thr protein kinase family. MAP2K4 phosphorylates MAP kinases in response to various environmental stresses or mitogenic stimuli. MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38 are substrates for MAP2K4, but MAPK1/ERK2 and MAPK3/ERK1 are not phosphorylated by MAP2K4. Structurally, MAP2K4 contains a kinase domain that is phosphorylated and activated by MAP3K1(aka MEKK1). MAP2K4 contains multiple amino acid sites that are phosphorylated and ubiquitinated. Genetic studies using Map2k4 knockout mice revealed embryonic lethality, impaired hepatogenesis and defective liver formation. Analysis of chimeric mice identified a role for Map2k4 in T cell cytokine production and proliferation. Map2k4-deficient chimeric mice frequently develop lymphadenopathy. MAP2K4 is altered in 1.97% of all human cancers.
Background References
1. Katzengruber L et al. MKK4 Inhibitors-Recent Development Status and Therapeutic Potential. Int J Mol Sci. 2023 Apr
2. Gu L et al. Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway. Biomed Pharmacother. 2023 Aug
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
c Jun N terminal kinase kinase 1 antibody
C-JUN N-terminal kinase kinase 1 antibody
Dual specificity mitogen activated protein kinase kinase 4 antibody
Dual specificity mitogen-activated protein kinase kinase 4 antibody
JNK Activated Kinase 1 antibody
JNK activating kinase 1 antibody
JNK-activating kinase 1 antibody
JNKK antibody
JNKK1 antibody
MAP kinase kinase 4 antibody
ExpandImages
-
Western blot analysis of MEK4 / MKK4 on different lysates with Rabbit anti-MEK4 / MKK4 antibody (HA723412) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: A431 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 46 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723412) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MEK4 / MKK4 antibody (HA723412) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723412) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MEK4 / MKK4 antibody (HA723412) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723412) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of 293T cells labeling MEK4 / MKK4.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723412, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
