MEK4 / MKK4 Recombinant Rabbit Monoclonal Antibody [PSH11-99]

Overview

Product Name

MEK4 / MKK4 Recombinant Rabbit Monoclonal Antibody [PSH11-99] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MEK4 / MKK4 aa 50-399.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 44 kDa

Positive Control

293T cell lysate, HeLa cell lysate, A431 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue, human skeletal muscle tissue, 293T.

Conjugation

unconjugated

Clone Number

PSH11-99

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:2,000

IHC-P
1:200

FC
1:1,000

Target

Function

Dual-specificity mitogen-activated protein kinase kinase 4 is an enzyme that in humans is encoded by the MAP2K4 gene. MAP2K4 encodes a dual-specificity kinase that belongs to the Ser/Thr protein kinase family. MAP2K4 phosphorylates MAP kinases in response to various environmental stresses or mitogenic stimuli. MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38 are substrates for MAP2K4, but MAPK1/ERK2 and MAPK3/ERK1 are not phosphorylated by MAP2K4. Structurally, MAP2K4 contains a kinase domain that is phosphorylated and activated by MAP3K1(aka MEKK1). MAP2K4 contains multiple amino acid sites that are phosphorylated and ubiquitinated. Genetic studies using Map2k4 knockout mice revealed embryonic lethality, impaired hepatogenesis and defective liver formation. Analysis of chimeric mice identified a role for Map2k4 in T cell cytokine production and proliferation. Map2k4-deficient chimeric mice frequently develop lymphadenopathy. MAP2K4 is altered in 1.97% of all human cancers.

Background References

1. Katzengruber L et al. MKK4 Inhibitors-Recent Development Status and Therapeutic Potential. Int J Mol Sci. 2023 Apr

2. Gu L et al. Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway. Biomed Pharmacother. 2023 Aug

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P45985 Human

SwissProt: P47809 Mouse

Entrez Gene: 287398 Rat

Synonyms

c Jun N terminal kinase kinase 1 antibody

C-JUN N-terminal kinase kinase 1 antibody

Dual specificity mitogen activated protein kinase kinase 4 antibody

Dual specificity mitogen-activated protein kinase kinase 4 antibody

JNK Activated Kinase 1 antibody

JNK activating kinase 1 antibody

JNK-activating kinase 1 antibody

JNKK antibody

JNKK1 antibody

MAP kinase kinase 4 antibody

Expand

Images

Western blot analysis of MEK4 / MKK4 on different lysates with Rabbit anti-MEK4 / MKK4 antibody (HA751432) at 1/2,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: A431 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 46 kDa

Exposure time: 59 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751432) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MEK4 / MKK4 antibody (HA751432) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751432) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MEK4 / MKK4 antibody (HA751432) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA751432) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 293T cells labeling MEK4 / MKK4.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA751432, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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