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Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]

Usd: 385

Specification

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Catalog# HA723482

Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]

Application

  • WB

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr18 and Ser19 of human MYL9.

Species Reactivity

Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 20 kDa

Positive Control

PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate.

Conjugation

unconjugated

Clone Number

PSH12-71

Reactivity Data

WB
Mouse
Tested Tested
Rat
Tested Tested
Human
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

Myosin regulatory light polypeptide 9 is a protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Iwamura C et al. Elevated Myl9 reflects the Myl9-containing microthrombi in SARS-CoV-2-induced lung exudative vasculitis and predicts COVID-19 severity. Proc Natl Acad Sci U S A. 2022 Aug

2. Matsushita K et al. Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. Cancer Rep (Hoboken). 2022 Oct

Subcellular Location

Cytoplasm, cytoskeleton, cell cortex.

UNIPROT

SwissProt: Q9CQ19 Mouse

SwissProt: Q64122 Rat

Synonyms

20 kDa myosin light chain antibody

Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody

LC20 antibody

MGC3505 antibody

MLC 2 antibody

MLC-2C antibody

MLC2 antibody

MLY 9 antibody

MRLC1 antibody

MYL9 antibody

Expand

Images

  • Western blot analysis of Phospho-MYL9 (T18 + S19) on different lysates with Rabbit anti-Phospho-MYL9 (T18 + S19) antibody (<a href="/products/HA723482" style="font-weight: bold;text-decoration: underline;">HA723482</a>) at 1/5,000 dilution.<br /><br />Lane 1: PC-12 cell lysate<br />Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate<br />Lane 3: C2C12 cell lysate<br />Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate<br />Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723482" style="font-weight: bold;text-decoration: underline;">HA723482</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-MYL9 (T18 + S19) on different lysates with Rabbit anti-Phospho-MYL9 (T18 + S19) antibody (HA723482) at 1/5,000 dilution.

    Lane 1: PC-12 cell lysate
    Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate
    Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723482) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"