Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]
Catalog# HA723482
Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]
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WB
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody [PSH12-71]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr18 and Ser19 of human MYL9.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate.
Conjugation
unconjugated
Clone Number
PSH12-71
Reactivity Data
| WB | |
|---|---|
| Mouse |
|
| Rat |
|
| Human |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
Myosin regulatory light polypeptide 9 is a protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Background References
1. Iwamura C et al. Elevated Myl9 reflects the Myl9-containing microthrombi in SARS-CoV-2-induced lung exudative vasculitis and predicts COVID-19 severity. Proc Natl Acad Sci U S A. 2022 Aug
2. Matsushita K et al. Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. Cancer Rep (Hoboken). 2022 Oct
Subcellular Location
Cytoplasm, cytoskeleton, cell cortex.
Synonyms
20 kDa myosin light chain antibody
Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
LC20 antibody
MGC3505 antibody
MLC 2 antibody
MLC-2C antibody
MLC2 antibody
MLY 9 antibody
MRLC1 antibody
MYL9 antibody
ExpandImages
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Western blot analysis of Phospho-MYL9 (T18 + S19) on different lysates with Rabbit anti-Phospho-MYL9 (T18 + S19) antibody (HA723482) at 1/5,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate
Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723482) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"