Phospho-MYL9 (T18 + S19) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH12-71] (HA751447)
Catalog# HA751447
Rabbit Monoclonal to Phospho-MYL9 (T18 + S19)
Tested for
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WB
Reacts with
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-MYL9 (T18 + S19) on different lysates with Rabbit anti-Phospho-MYL9 (T18 + S19) antibody (HA751447) at 1/5,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate
Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751447) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: PC-12 cell lysate
Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate
Lane 5: C2C12 starved overnight then add 100nM Calyculin A for 30 minutes then treated with 100ng/mL insulin for 20 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751447) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.