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Phospho-PLC-gamma-1 (S1248) Recombinant Rabbit Monoclonal Antibody [PSH13-62]

Usd: 385

Specification

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Catalog# HA723551

Phospho-PLC-gamma-1 (S1248) Recombinant Rabbit Monoclonal Antibody [PSH13-62]

Application

  • WB

  • IP

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-PLC-gamma-1 (S1248) Recombinant Rabbit Monoclonal Antibody [PSH13-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser1248 of human PLC-gamma-1.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 149 kDa

Positive Control

A431 cell lysate, A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate.

Conjugation

unconjugated

Clone Number

PSH13-62

Reactivity Data

WB IP
Human
Tested Tested
Tested Tested
Rat
Tested
Mouse
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:10,000

  • IP

  • 1-2μg/sample

Target

Function

Phospholipase C, gamma 1, also known as PLCG1 and PLCgamma1, is a protein that in humans involved in cell growth, migration, apoptosis, and proliferation. It is encoded by the PLCG1 gene and is part of the PLC superfamily. PLCγ1 is a cell growth factor from the PLC superfamily. PLCγ1 is used during the cell growth and in a cell migration and apoptosis, all of which are vital cell processes that, if disrupted by mutations, can cause cancerous cells to form within the body. Mutations in this protein show an increase in issues in cells regarding regulation of proliferation and their cell signaling. PLCγ1 roles are also involved in neuronal actin growth, calcium signaling, and brain development. It is highly regulated by multiple factors, such as PIK3, AMPK, and FAK. It is part of the PIP3 pathway and leads to and increase in calcium in the cells. In neuronal cells, PLCγ1 is highly involved in actin cytoskeleton organization and synaptic plasticity.

Background References

1. Tao P et al. A gain-of-function variation in PLCG1 causes a new immune dysregulation disease. J Allergy Clin Immunol. 2023 Nov

2. Schnoeder TM et al. PLCG1 is required for AML1-ETO leukemia stem cell self-renewal. Blood. 2022 Feb

Subcellular Location

Cell projection, lamellipodium, ruffle.

UNIPROT

SwissProt: P19174 Human

Synonyms

1 phosphatidyl D myo inositol 4 5 bisphosphate antibody

1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody

Inositoltrisphosphohydrolase antibody

Monophosphatidylinositol phosphodiesterase antibody

NCKAP3 antibody

Phosphatidylinositol phospholipase C antibody

Phosphoinositidase C antibody

Phosphoinositide phospholipase C antibody

Phosphoinositide phospholipase C-gamma-1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody (<a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a>) at 1/2,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate<br />Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 149 kDa<br />Observed band size: 149 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-PLC-gamma-1 (S1248) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (S1248) antibody (HA723551) at 1/2,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate
    Lane 3: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 149 kDa
    Observed band size: 149 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723551) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with <a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a> at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input)<br />Lane 2: <a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a> IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723551" style="font-weight: bold;text-decoration: underline;">HA723551</a> in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 7 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ☑ Cell treatment (CT)

    Phospho-PLC-gamma-1 (S1248) was immunoprecipitated from 0.2 mg A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate with HA723551 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723551 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate (input)
    Lane 2: HA723551 IP in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate
    Lane 3: Rabbit IgG instead of HA723551 in A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 7 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation