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Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02]

Usd: 385

Specification

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Catalog# HA723592

Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human BCKDHA.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB

Molecular Weight

Predicted band size: 50 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

PSH14-02

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested
Green Monkey
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:20,000

Target

Function

A 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial is an enzyme that in humans is encoded by the BCKDHA gene. BCKDHA is a coding gene that is part of the BCKD complex (branched-chain alpha-keto acid dehydrogenase). The second major step in the catabolism of the branched-chain amino acids (isoleucine, leucine, and valine) is catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that consists of 3 catalytic components: a heterotetrameric (alpha2, beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase. The BCKDHA gene encodes the alpha subunit of E1, and the BCKDHB gene (MIM 248611) encodes the beta subunit of E1.

Background References

1. Tian Y et al. BCKDHA contributes to melanoma progression by promoting the expressions of lipogenic enzymes FASN and ACLY. Exp Dermatol. 2023 Oct

2. Zhou F et al. BCKDH kinase promotes hepatic gluconeogenesis independent of BCKDHA. Cell Death Dis. 2024 Oct

Subcellular Location

Mitochondrion matrix.

UNIPROT

SwissProt: P12694 Human

SwissProt: P50136 Mouse

SwissProt: P11960 Rat

Synonyms

Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody

FLJ45695 antibody

OVD1A antibody

2 oxoisovalerate dehydrogenase (lipoamide) antibody

2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody

BCKDE1A antibody

BCKDH E1 alpha antibody

BCKDH E1-alpha antibody

BCKDHA antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (<a href="/products/HA723592" style="font-weight: bold;text-decoration: underline;">HA723592</a>) at 1/20,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/Lane)<br />Lane 2: HepG2 cell lysate (10 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (10 µg/Lane)<br />Lane 4: C6 cell lysate (10 µg/Lane)<br />Lane 5: COS-1 cell lysate (10 µg/Lane)<br />Lane 6: Mouse liver tissue lysate (20 µg/Lane)<br />Lane 7: Rat liver tissue lysate (20 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723592" style="font-weight: bold;text-decoration: underline;">HA723592</a>) at 1/20,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA723592) at 1/20,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/Lane)
    Lane 2: HepG2 cell lysate (10 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
    Lane 4: C6 cell lysate (10 µg/Lane)
    Lane 5: COS-1 cell lysate (10 µg/Lane)
    Lane 6: Mouse liver tissue lysate (20 µg/Lane)
    Lane 7: Rat liver tissue lysate (20 µg/Lane)
    Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 48 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723592) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"