Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH14-02] (HA751498)
Catalog# HA751498
Rabbit Monoclonal to Phospho--BCKDHA (S293)
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA751498) at 1/20,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: C6 cell lysate (10 µg/Lane)
Lane 5: COS-1 cell lysate (10 µg/Lane)
Lane 6: Mouse liver tissue lysate (20 µg/Lane)
Lane 7: Rat liver tissue lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 48 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751498) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA751498) at 1/20,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: C6 cell lysate (10 µg/Lane)
Lane 5: COS-1 cell lysate (10 µg/Lane)
Lane 6: Mouse liver tissue lysate (20 µg/Lane)
Lane 7: Rat liver tissue lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 48 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751498) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.