Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02] - BSA and Azide free
Catalog# HA751498
Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
Phospho-BCKDHA (S293) Recombinant Rabbit Monoclonal Antibody [PSH14-02] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human BCKDHA.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
PSH14-02
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:20,000
Target
Function
A 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial is an enzyme that in humans is encoded by the BCKDHA gene. BCKDHA is a coding gene that is part of the BCKD complex (branched-chain alpha-keto acid dehydrogenase). The second major step in the catabolism of the branched-chain amino acids (isoleucine, leucine, and valine) is catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that consists of 3 catalytic components: a heterotetrameric (alpha2, beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2; MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3; MIM 238331). The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase. The BCKDHA gene encodes the alpha subunit of E1, and the BCKDHB gene (MIM 248611) encodes the beta subunit of E1.
Background References
1. Tian Y et al. BCKDHA contributes to melanoma progression by promoting the expressions of lipogenic enzymes FASN and ACLY. Exp Dermatol. 2023 Oct
2. Zhou F et al. BCKDH kinase promotes hepatic gluconeogenesis independent of BCKDHA. Cell Death Dis. 2024 Oct
Subcellular Location
Mitochondrion matrix.
Synonyms
Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
FLJ45695 antibody
OVD1A antibody
2 oxoisovalerate dehydrogenase (lipoamide) antibody
2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
BCKDE1A antibody
BCKDH E1 alpha antibody
BCKDH E1-alpha antibody
BCKDHA antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA751498) at 1/20,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: C6 cell lysate (10 µg/Lane)
Lane 5: COS-1 cell lysate (10 µg/Lane)
Lane 6: Mouse liver tissue lysate (20 µg/Lane)
Lane 7: Rat liver tissue lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 48 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751498) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"