Biotin Conjugated Mouse IL-7 Recombinant Rabbit Monoclonal Antibody [PSH14-30] - Detector
Overview
Product Name
Biotin Conjugated Mouse IL-7 Recombinant Rabbit Monoclonal Antibody [PSH14-30] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IL-7 aa 26-154 (HA211019).
Species Reactivity
Mouse
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Mouse IL-7 protein (HA211019).
Conjugation
Biotin
Clone Number
PSH14-30
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-29] to Mouse IL-7 antibody (Capture) (HA723621) and Recombinant Mouse IL-7 protein (HA211019) as the standard. The reference range value is 15.6-2,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.
Background References
1. von Freeden-Jeffry U., Vieira P., Lucian L.A., McNeil T., Burdach S.E., Murray R. Lymphopenia in interleukin (IL)-7 gene-deleted mice identifies IL-7 as a nonredundant cytokine. J. Exp. Med. 181:1519-1526 (1995)
2. Plumb A.W., Sheikh A., Carlow D.A., Patton D.T., Ziltener H.J., Abraham N. Interleukin-7 in the transition of bone marrow progenitors to the thymus. Immunol. Cell Biol. 95:916-924 (2017)
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 7 antibody
IL-7 antibody
Il7 antibody
IL7_HUMAN antibody
Interleukin 7 antibody
Interleukin-7 antibody
LP-1 antibody
Lymphopoietin 1 antibody
Images
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![Sandwich ELISA analysis of Mouse IL-7 matched pair antibodies<br /><br />Capture: <a href="/products/HA723621" style="font-weight: bold;text-decoration: underline;">HA723621</a>, Mouse IL-7 Rabbit mAb [PSH14-29]<br />Detector: <a href="/products/HA723622" style="font-weight: bold;text-decoration: underline;">HA723622</a>, Mouse IL-7 Rabbit mAb [PSH14-30]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723621" style="font-weight: bold;text-decoration: underline;">HA723621</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-7 protein (<a href="/products/HA211019" style="font-weight: bold;text-decoration: underline;">HA211019</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723622" style="font-weight: bold;text-decoration: underline;">HA723622</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723623B_1.png?v=20260604174805)
Sandwich ELISA analysis of Mouse IL-7 matched pair antibodies
Capture: HA723621, Mouse IL-7 Rabbit mAb [PSH14-29]
Detector: HA723622, Mouse IL-7 Rabbit mAb [PSH14-30]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723621) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-7 protein (HA211019) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723622, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of spiked IL-7 in mouse cell culture media samples.<br /><br />Capture: <a href="/products/HA723621" style="font-weight: bold;text-decoration: underline;">HA723621</a>, Mouse IL-7 Rabbit mAb [PSH14-29]<br />Detector: <a href="/products/HA723622" style="font-weight: bold;text-decoration: underline;">HA723622</a>, Mouse IL-7 Rabbit mAb [PSH14-30]<br /><br />The concentrations of IL-7 were measured in duplicates, interpolated from the IL-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723623B_2.png?v=20260604174805)
Interpolated concentrations of spiked IL-7 in mouse cell culture media samples.
Capture: HA723621, Mouse IL-7 Rabbit mAb [PSH14-29]
Detector: HA723622, Mouse IL-7 Rabbit mAb [PSH14-30]
The concentrations of IL-7 were measured in duplicates, interpolated from the IL-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"