Human EphA2 Recombinant Rabbit Monoclonal Antibody [PSH15-13] - BSA and Azide free (Detector)
Overview
Product Name
Human EphA2 Recombinant Rabbit Monoclonal Antibody [PSH15-13] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human EPHA2 aa 24-534 (HA211075).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human EphA2 protein (HA211075).
Conjugation
unconjugated
Clone Number
PSH15-13
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-12] to Human EphA2 antibody (Capture) (HA723714) and Recombinant Human EphA2 protein (HA211075) as the standard. The reference range value is 7.8-2,000 pg/mL.
Target
Function
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
Background References
1. Xiao T et al. Targeting EphA2 in cancer. J Hematol Oncol. 2020 Aug
2. Wilson K et al. Oncogenic functions and therapeutic targeting of EphA2 in cancer. Oncogene. 2021 Apr
Subcellular Location
Cell membrane, Cell projection, ruffle membrane, lamellipodium membrane, Cell junction, focal adhesion.
UNIPROT
Synonyms
ARCC2 antibody
AW545284 antibody
CTPA antibody
CTPP1 antibody
CTRCT6 antibody
EC 2.7.10.1 antibody
Eck antibody
Eph receptor A2 antibody
EPHA2 antibody
EPHA2_HUMAN antibody
ExpandImages
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![Sandwich ELISA analysis of Human EphA2 matched pair antibodies<br /><br />Capture: <a href="/products/HA723714" style="font-weight: bold;text-decoration: underline;">HA723714</a>, Human EphA2 Rabbit mAb [PSH15-12]<br />Detector: <a href="/products/HA723715" style="font-weight: bold;text-decoration: underline;">HA723715</a>, Human EphA2 Rabbit mAb [PSH15-13]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723714" style="font-weight: bold;text-decoration: underline;">HA723714</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (<a href="/products/HA211075" style="font-weight: bold;text-decoration: underline;">HA211075</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723715" style="font-weight: bold;text-decoration: underline;">HA723715</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723715_1.png?v=20260401002455)
Sandwich ELISA analysis of Human EphA2 matched pair antibodies
Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12]
Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723714) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (HA211075) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723715, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA723714" style="font-weight: bold;text-decoration: underline;">HA723714</a>, Human EphA2 Rabbit mAb [PSH15-12]<br />Detector: <a href="/products/HA723715" style="font-weight: bold;text-decoration: underline;">HA723715</a>, Human EphA2 Rabbit mAb [PSH15-13]<br /><br />The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract.](https://storage.huabio.cn/huabio/productImg/HA723715_2.png?v=20260401002455)
Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load.
Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12]
Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13]
The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract. -
![Interpolated concentrations of spiked EphA2 in human cell culture media samples.<br /><br />Capture: <a href="/products/HA723714" style="font-weight: bold;text-decoration: underline;">HA723714</a>, Human EphA2 Rabbit mAb [PSH15-12]<br />Detector: <a href="/products/HA723715" style="font-weight: bold;text-decoration: underline;">HA723715</a>, Human EphA2 Rabbit mAb [PSH15-13]<br /><br />The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723715_3.png?v=20260401002455)
Interpolated concentrations of spiked EphA2 in human cell culture media samples.
Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12]
Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13]
The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Biotin Conjugated Human EphA2 Recombinant Rabbit Monoclonal Antibody [PSH15-13] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human
Conjugate: Biotin
