Prosurfactant Protein C Recombinant Rabbit Monoclonal Antibody [PSH15-28]
Overview
Product Name
Prosurfactant Protein C Recombinant Rabbit Monoclonal Antibody [PSH15-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Prosurfactant Protein C aa 1-50.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, FC, IF-Tissue
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
Mouse lung tissue lysate, Rat lung tissue lysate, human lung tissue, mouse lung tissue, rat lung tissue, HeLa cells transfected with Prosurfactant Protein C, 293T cells transfected with Prosurfactant Protein C.
Conjugation
unconjugated
Clone Number
PSH15-28
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:3,000
-
IF-Cell
-
1:2,000
-
FC
-
1:1,000
-
IF-Tissue
-
1:1,000
Target
Function
Surfactant protein C (SP-C), is one of the pulmonary surfactant proteins. In humans this is encoded by the SFTPC gene. It is a membrane protein. SFTPC is a 197-residue protein made up of two halves: a unique N-terminal propeptide domain and a C-terminal BRICHOS domain. The around 100-aa long propeptide domain actually contains not only the cleaved part, but also the mature peptide. The propeptide of pulmonary surfactant C has an N-terminal alpha-helical segment whose suggested function was stabilization of the protein structure, since the mature peptide can irreversibly transform from its native alpha-helical structure to beta-sheet aggregates and form amyloid fibrils. The correct intracellular trafficking of proSP-C has also been reported to depend on the propeptide. The structure of the BRICHOS domain has been solved. Mutations in this domain also lead to amyloid fibrils made up of the mature peptide, suggesting a chaperone activity.
Background References
1. Abdel Megeid AK et al. Correlating SFTPC gene variants to interstitial lung disease in Egyptian children. J Genet Eng Biotechnol. 2022 Aug
2. Mou H. Induced Pluripotent Stem Cell-derived Alveolar Type II Heterogeneity: Revealed by SFTPC Expression. Am J Respir Cell Mol Biol. 2021 Oct
Subcellular Location
Secreted, extracellular space, surface film.
Synonyms
BRICD6 antibody
BRICHOS domain containing 6 antibody
PSP C antibody
PSPC antibody
PSPC_HUMAN antibody
Pulmonary surfactant apoprotein 2 antibody
Pulmonary surfactant apoprotein PSP C antibody
pulmonary surfactant apoprotein-2 SP-C antibody
Pulmonary surfactant associated protein C antibody
Pulmonary surfactant associated proteolipid SPL pVal antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Prosurfactant Protein C on different lysates with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/5,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse spleen tissue lysate (negative)
Lane 3: Rat lung tissue lysate
Lane 4: Rat spleen tissue lysate (negative)
Lysates/proteins at 40 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723734) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Prosurfactant Protein C on different lysates with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/5,000 dilution.
Lane 1: 293T transfected with empty control cell lysate
Lane 2: 293T transfected with Prosurfactant Protein C cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723734) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue (negative) with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723734) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells transfected with or without Prosurfactant Protein C labeling Prosurfactant Protein C with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Prosurfactant Protein C antibody (HA723734) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with Prosurfactant Protein C (right) labeling Prosurfactant Protein C.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723734, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-Tissue
Species: Mouse
Site: lung
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000
Citation
-
Engineered mesenchymal stem cells regulate integrated stress response for fibrosis therapy
Journal: Med
DOI: 10.1016/j.medj.2026.101261
IF: 13.3
Application: WB
Reactivity: Mouse
Publish date: 2026 Aug
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"